VSV-G Epitope Tag Antibody - BSA Free

Western Blot: VSV-G Epitope Tag Antibody [NB100-2485] - 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibody used at 0.04 ug/ml (1:25,000).

• Reactivity: ET
• Applications: WB, Simple Western, ELISA, ICC/IF, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

VSV-G Epitope Tag Antibody - BSA Free Summary

• Immunogen: Synthetic peptide: YTDIEMNRLGK conjugated to KLH.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:1000 - 1:30000
  • Immunocytochemistry/ Immunofluorescence 1:100-1:400
  • Immunoprecipitation 1 - 4 ug/mg lysate
  • Simple Western 1:30 - 1:270
  • Western Blot 1:1000-1:30000
• Application Notes: ELISA 1:1,000 - 1:30,000; for coating plates 1:100 - 1:500

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for VSV-G Epitope Tag Antibody - BSA Free

• Vesicular stomatitis virus glycoprotein tag
• VSV epitope tag
• VSV tag
• VSV-G epitope tag
• vsvg tag
• VSVG

Background

The VSV-G epitope tag is commonly engineered onto the N- or C- terminus of a protein of interest so that the tagged protein can be analyzed and visualized using immunochemical methods. The recognized VSV-G epitope represents the amino acid sequence YTDIEMNRLGK derived from the Vesicular Stomatitis viral glycoprotein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for VSV-G Epitope Tag Antibody (NB100-2485)(11)

Publications using NB100-2485

• Ilahibaks NF, Ardisasmita AI, Xie S et al. TOP-EVs: Technology of Protein delivery through Extracellular Vesicles is a versatile platform for intracellular protein delivery Journal of controlled release : official journal of the Controlled Release Society 2023-02-15 [PMID: 36746337] (WB)
• Ilahibaks N, Roefs M, Brans M et al. Extracellular vesicle?mediated protein delivery to the liver Journal of Extracellular Biology 2023-09-01 (WB)
• Bin Yu, Shoeb Ikhlas, Chunsheng Ruan, Xingxing Zhong, Dongsheng Cai Innate and Adaptive Immunity of Murine Neural Stem Cell-Derived piRNA Exosomes/Microvesicles against Pseudotyped SARS-CoV-2 and HIV-Based Lentivirus iScience 2020-11-13 [PMID: 33205008]
• Ong YS, Tang BL, Loo LS et al. p125A exists as part of the mammalian Sec13/Sec31 COPII subcomplex to facilitate ER-Golgi transport. J Cell Biol 2010-08-01 [PMID: 20679433]
• Jernigan KK, Cselenyi CS, Thorne CA et al. Gbetagamma activates GSK3 to promote LRP6-mediated beta-catenin transcriptional activity. Sci Signal 2010-05-01 [PMID: 20460648]
• Hossain MM, Hosono-Fukao T, Tang R et al. Direct detection of HSulf-1 and HSulf-2 activities on extracellular heparan sulfate and their inhibition by PI-88. Glycobiology 2010-02-01 [PMID: 19822709]
• Kathiresan T, Harvey M, Orchard S et al. A protein interaction network for the large conductance Ca(2+)-activated K(+) channel in the mouse cochlea. Mol Cell Proteomics 2009-08-01 [PMID: 19423573]
• Rendl LM, Bieman MA, Smibert CA et al. S. cerevisiae Vts1p induces deadenylation-dependent transcript degradation and interacts with the Ccr4p-Pop2p-Not deadenylase complex. RNA 2008-07-01 [PMID: 18469165]
• Roberts TM, Zaidi IW, Vaisica JA et al. Regulation of rtt107 recruitment to stalled DNA replication forks by the cullin rtt101 and the rtt109 acetyltransferase. Mol Biol Cell 2008-01-01 [PMID: 17978089]
• Chai N, Chang HE, Nicolas E et al. Assembly of hepatitis B virus envelope proteins onto a lentivirus pseudotype that infects primary human hepatocytes. J Virol 2007-10-01 [PMID: 17670822]
• Roberts TM, Kobor MS, Bastin-Shanower SA et al. Slx4 regulates DNA damage checkpoint-dependent phosphorylation of the BRCT domain protein Rtt107/Esc4. Mol Biol Cell 2006-01-01 [PMID: 16267268]