VSTM5 Antibody (2696A) [Unconjugated]

HEK293 human embryonic kidney cell line transfected with (A) human VSTM5 or (B) irrelevant protein, and eGFP was stained with Rabbit Anti-Human VSTM5 Monoclonal Antibody (Catalog # MAB10872) followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (F0111). Quadrant markers were set based on Rabbit IgG Control Antibody (MAB1050). Staining was performed using our Staining Membrane-associated Proteins protocol.

VSTM5 was detected in immersion fixed paraffin-embedded sections of human brain (hypothalamus) using Rabbit Anti-Human/Mouse VSTM5 Monoclonal Antibody (Catalog # MAB10872) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cell bodies. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

• Reactivity: Hu
• Applications: Flow, IHC
• Clone: 2696A
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

VSTM5 Antibody (2696A) [Unconjugated] Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Human embryonic kidney cell HEK293-derived human VSTM5
  Met1-His147
  Accession # NP_001138343
• Specificity: Detects human VSTM5 in direct ELISAs.
• Source: N/A
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Purity Statement: Protein A or G purified from cell culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunohistochemistry 3-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for VSTM5 Antibody (2696A) [Unconjugated]

• C11orf90
• V-set and transmembrane domain containing 5
• V-set and transmembrane domain-containing protein 5
• VSTM5

Background

V-set and transmembrane domain-containing protein 5 (VSTM5) is a cell-adhesion like molecule that belongs to the Ig superfamily.  This molecule is expressed in hippocampal neurons.  It regulates neuronal morphology by promoting dendritic protrusions that develop into dendritic spines.  VSTM5 is a novel factor involved in the modulation of the neuronal membrane and a critical element in normal neuronal circuit formation.  It is one of the target genes responsible for variations in patient responses to treatments for major depressive disorder. Overexpression of VSTM5 in utero delays neuronal migration and induces multiple branches in leading processes during corticogenesis.  Glycosylation at individual N-linked glycosylation sites (Asn43, Asn87, Asn101, and Asn108) not only play essential roles in surface expression of VSTM5 but also in the formation of neuronal dendritic filopodia.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • NP_001138343()