VSTM2A Antibody (1024718) [Unconjugated]

Recombinant Human VSTM2A protein was serially diluted 2-fold and captured by Mouse Anti-Human VSTM2A Monoclonal Antibody (Catalog # MAB10037) coated on a Clear Polystyrene Microplate (DY990). Mouse Anti-Human VSTM2A Monoclonal Antibody (MAB100371) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY998) followed by Substrate Solution (DY999) and stopping the enzymatic reaction with Stop Solution (DY994).

• Reactivity: Hu
• Applications: ELISA
• Clone: 1024718
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

VSTM2A Antibody (1024718) [Unconjugated] Summary

• Immunogen: Human embryonic kidney cell HEK293-derived human VSTM2A
  Ser25-Phe244
  Accession # Q8TAG5-2
• Specificity: Detects human VSTIM-2A in direct ELISAs.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for VSTM2A Antibody (1024718) [Unconjugated]

• MGC33530
• V-set and transmembrane domain containing 2
• V-set and transmembrane domain containing 2A
• V-set and transmembrane domain-containing protein 2A
• VSTM2
• VSTM2A

Background

V-set and transmembrane domain-containing protein 2A (VSTM2A) is a secreted glycoprotein that is expressed by committed preadipocytes. N-linked glycosylation is crucial for its secretion, but not for preadipocyte cell differentiation activity. It is expressed during adipocyte development and its over-expression promotes adipogenesis (1). VSTM2A is highly expressed in the brain and Vstm2a was identified as an enigmatic gene that is highly produced in mouse brain (1, 2). A positive association has been observed between Vstm2a and Pparg2. VSTM2A plays a role in the regulation of the early stage of white and brown preadipocyte cell differentiation. It promotes adipogenic commitment of preadipocytes by increasing gene expression of the transcription factor PPARG in a BMP4-dependent signaling pathway (1, 3). In humans, two isoforms (1 and 2) exist due to alternative splicing. Human VSTM2A is synthesized either as a 236 amino acid (aa) (Isoform 1) or a 244 aa (Isoform 2) precursor that contains a 24 aa signal sequence followed by the VSTM2A domain. Human Isoform 2 VSTM2A shares 78% aa sequence identity with mouse and rat VSTM2A.

1. Secco, B. et al. (2017) Cell Rep. 18:93.
2. Pandey, A.K. et al. (2014) PloS One. 9:e88889.
3. Berry, D.C. et al. (2013) Development. 140:3939.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • Q8TAG5-2()