Virilizer Antibody - Azide and BSA Free

Western Blot: Virilizer Antibody - Azide and BSA Free [NBP2-93159] - Western blot analysis of lysates from Mouse lung, using Virilizer Rabbit pAb at 1:2000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.

Immunohistochemistry-Paraffin: Virilizer Antibody [NBP2-93159] - Mouse kidney using KIAA1429 Rabbit pAb at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunohistochemistry: Virilizer Antibody - Azide and BSA Free [NBP2-93159] - Immunohistochemistry analysis of paraffin-embedded human esophageal cancer using Virilizer Rabbit pAb at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Virilizer Antibody - Azide and BSA Free Summary

• Description: Novus Biologicals Rabbit Virilizer Antibody - Azide and BSA Free (NBP2-93159) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. Anti-Virilizer Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1400-1700 of human KIAA1429 (NP_056311.2). FMRQILNSDTIGCCGDDNGLMEVEGAHTSRTMSINAAELKQLLQSKEESPENLFLELEKLVLEHSKDDDNLDSLLDSVVGLKQMLESSGDPLPLSDQDVEPVLSAPESLQNLFNNRTAYVLADVMDDQLKSMWFTPFQAEEIDTDLDLVKVDLIELSEKCCSDFDLHSELERSFLSEPSSPGRTKTTKGFKLGKHKHETFITSSGKSEYIEPAKRAHVVPPPRGRGRGGFGQGIRPHDIFRQRKQNTSRPPSMHVDDFVAAESKEVVPQDGIPPPKRPLKVSQKISSRGGFSGNRGGRGAF
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: VIRMA
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:100
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin
  • Western Blot 1:1000 - 1:5000
• Theoretical MW: 202 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.05% Proclin 300
• Purity: Affinity purified

Alternate Names for Virilizer Antibody - Azide and BSA Free

• DKFZP434I116
• DKFZp781B2117
• fSAP121
• functional spliceosome-associated protein 121
• KIAA1429
• MGC138493
• MGC141940
• protein virilizer homolog

Background

The virilizer (vir) gene was discovered as a factor required for dosage compensation and sex determination in female flies. In drosophila it has been determined to function as a positive regulator of female-specific mRNA splicing. Database searches using the deduced amino acid sequence of the drosophila VIR retrieved a human protein BAA92667 (GenBank) which corresponds to the gene designated as KIAA1429. The human protein is 28% identical and about 40% similar to drosophila VIR and contains the putative transmembrane domain of drosophila VIR.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Virilizer Antibody (NBP2-93159)(1)

Publication using NBP2-93159

• Donghua Zou, Xiaohua Huang, Yating Lan, Mika Pan, Jieqiong Xie, Qi Huang, Jingyi Zeng, Chun Zou, Zifei Pei, Cuihua Zou, Yingwei Mao, Jiefeng Luo Single-cell and spatial transcriptomics reveals that PTPRG activates the m 6 A methyltransferase VIRMA to block mitophagy-mediated neuronal death in Alzheimer's disease. Pharmacological research 2024-03-04 [PMID: 38325728]

Bioinformatics

• Gene Symbol: VIRMA