Virilizer Antibody - BSA Free

Images

 
Immunocytochemistry/ Immunofluorescence: Virilizer Antibody [NBP1-85118] - Staining of human cell line U-2 OS shows localization to nucleoplasm, nuclear bodies & cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Virilizer Antibody [NBP1-85118] - Staining of human testis shows moderate to strong nuclear positivity in cells in seminiferous ducts.
Immunohistochemistry-Paraffin: Virilizer Antibody [NBP1-85118] - Staining of human rectum shows moderate nuclear positivity in glandular cells.
Immunohistochemistry-Paraffin: Virilizer Antibody [NBP1-85118] - Staining of human skin shows moderate nuclear positivity in squamous epithelial cells.
Immunohistochemistry-Paraffin: Virilizer Antibody [NBP1-85118] - Staining of human skeletal muscle shows very weak cytoplasmic positivity in myocytes.
Immunohistochemistry-Paraffin: Virilizer Antibody [NBP1-85118] - Staining of human endometrium shows moderate to strong nuclear positivity in glandular cells.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free

Order Details

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Catalog# & Formulation Size Price

Virilizer Antibody - BSA Free Summary

Description
Novus Biologicals Rabbit Virilizer Antibody - BSA Free (NBP1-85118) is a polyclonal antibody validated for use in IHC and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: LSGRLDSDEQKIQNDIIDILLTFTQGVNEKLTISEETLANNTWSLMLKEVLSSILKVPEGFFSGLILLSELLPLPLPMQTTQVIEPHDISVALNTRKL
Predicted Species
Mouse (100%), Rat (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
VIRMA
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:20 - 1:50
  • Immunohistochemistry-Paraffin 1:20 - 1:50
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
Virilizer Protein (NBP1-85118PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Virilizer Antibody - BSA Free

  • DKFZP434I116
  • DKFZp781B2117
  • fSAP121
  • functional spliceosome-associated protein 121
  • KIAA1429
  • MGC138493
  • MGC141940
  • protein virilizer homolog

Background

The virilizer (vir) gene was discovered as a factor required for dosage compensation and sex determination in female flies. In drosophila it has been determined to function as a positive regulator of female-specific mRNA splicing. Database searches using the deduced amino acid sequence of the drosophila VIR retrieved a human protein BAA92667 (GenBank) which corresponds to the gene designated as KIAA1429. The human protein is 28% identical and about 40% similar to drosophila VIR and contains the putative transmembrane domain of drosophila VIR.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol VIRMA