USH1C Overexpression Lysate (Native)

Images

 
Western Blot: USH1C Overexpression Lysate (Adult Normal) [NBP2-05243] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for USH1C.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

USH1C Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC215316 and based on accession number NP_710142. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens USH1C, mRNA.
Gene
USH1C

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
101.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for USH1C

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for USH1C Overexpression Lysate (Native)

  • AIE75
  • AIE-75
  • Antigen NY-CO-38/NY-CO-37
  • Autoimmune enteropathy-related antigen AIE-75
  • deafness, autosomal recessive 18
  • DFNB18
  • harmonin
  • NY-CO-37
  • NY-CO-38
  • PDZ-45
  • PDZ73
  • PDZ-73
  • PDZ-73/NY-CO-38
  • Protein PDZ-73
  • Renal carcinoma antigen NY-REN-3
  • ush1cpst
  • Usher syndrome 1C (autosomal recessive, severe)
  • Usher syndrome type-1C protein

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP1-84266
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P
NBP1-85704
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
AF6729
Species: Hu
Applications: WB, IHC, ICC
NBP1-89076
Species: Hu
Applications: IHC, IHC-P
42720002
Species: Ze
Applications: WB, ELISA, ICC/IF, IHC, IHC-Fr
NLS1729
Species: Hu, Eq
Applications: WB, IHC-P
H00025861-M05
Species: Hu
Applications: WB, ELISA, ICC/IF
H00009223-M03
Species: Hu, Rt, Ca, Ha
Applications: WB, ELISA, ICC/IF
NBP1-85885
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
H00079955-P01
Species: Hu
NBP1-85047
Species: Hu, Po
Applications: Simple Western, ICC/IF, IHC, IHC-P, ICC
NB300-556
Species: Hu, Mu, Rt, In, Pm
Applications: WB, ChIP, B/N, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP

Publications for USH1C Lysate (NBP2-05243) (0)

There are no publications for USH1C Lysate (NBP2-05243).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for USH1C Lysate (NBP2-05243) (0)

There are no reviews for USH1C Lysate (NBP2-05243). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for USH1C Lysate (NBP2-05243) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional USH1C Products

Bioinformatics Tool for USH1C Lysate (NBP2-05243)

Discover related pathways, diseases and genes to USH1C Lysate (NBP2-05243). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for USH1C Lysate (NBP2-05243)

Discover more about diseases related to USH1C Lysate (NBP2-05243).
 

Pathways for USH1C Lysate (NBP2-05243)

View related products by pathway.

Research Areas for USH1C Lysate (NBP2-05243)

Find related products by research area.

Blogs on USH1C.

Auditory Infographic: Can you hear me now?
The auditory process involves several structures of the ear to convert sound waves into information that is processed by our brain. Learn more about the auditory process in our infographic below.Novus Biologicals offers reagents mentioned in the i...  Read full blog post.

Customers Who Bought This Also Bought

USH1C Antibody
NBP1-89189

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our USH1C Overexpression Lysate (Native) and receive a gift card or discount.

Bioinformatics

Gene Symbol USH1C