Trypsinogen Antibody [HRP]

Western Blot: Trypsinogen Antibody [HRP] [NBP1-77697] - Lane 1: Trypsinogen. Lane 2: None. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Peroxidase rabbit secondary antibody at 1:1,000 for 60 min at RT. Block: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 24 kDa, 24 kDa for Trypsinogen. Other band(s): None.

• Reactivity: Bv
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: HRP

Trypsinogen Antibody [HRP] Summary

• Description: For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
  
  This antibody (RABBIT) Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Trypsinogen [Bovine Pancreas]
• Immunogen: Trypsinogen [Bovine Pancreas] (Uniprot: Q29463)
• Localization: Secreted protein; extracellular space
• Specificity: Cross reactivity against Trypsinogen from other tissues and species may occur but have not been specifically determined.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Purity: Multi-step

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000 - 1:40000
  • Western Blot 1:1000 - 1:5000
• Application Notes: This product has been tested by ELISA and Western blot. This product is assayed against 1.0 ug of Trypsinogen [Bovine Pancreas] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:3,000 of the reconstitution concentration is suggested for this product. Specific conditions should be optimized by user.
  
  NOTE: Do NOT add Sodium Azide (it inhibits HRP irreversibly).
• Readout System:
  • NovaLume Plus Chemiluminescent Reagent LUM-555

Reactivity Notes

Cross reactivity against Trypsinogen from other tissues and species may occur but have not been specifically determined

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
• Preservative: 0.01% Gentamicin Sulfate
• Purity: Multi-step

Alternate Names for Trypsinogen Antibody [HRP]

• 2
• Brain trypsinogen
• Cationic Trypsinogen
• Mesotrypsinogen
• Pancreatic anionic trypsinogen
• Protease
• PRSS2
• PRSS3
• PRSS4
• serine
• TRP 1
• TRP1
• TRY1
• TRY2
• TRY3. TRY4
• TRYP1
• TRYP2
• TRYP8
• Trypsin 2
• Trypsin 3
• Trypsin III
• Trypsin IV

Background

Trypsinogen has been shown to have certain intrinsic activity. It is usually considered, however, as the inactive precursor of trypsin which may be activated by removal of a terminal hexapeptide to yield single chain b trypsin. Subsequent limited autolysis produces other active forms having two or more peptide chains bound by disulfide bonds. The predominant forms are a trypsin, having two peptide chains and b, a single chain. Different activity and thermal stability are shown by a and b trypsin.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • Q29463(Bovine)