Trypsin Inhibitor Antibody - BSA Free

Western Blot: Trypsin Inhibitor Antibody [NBP1-78088] - Analysis using the Biotin conjugate of NBP1-78088. Detection of Lane 1: ~1 ug of purified Soybean Trypsin Inhibitor Reduced. Lane 2: 0.25 ug of purified Soybean Trypsin Inhibitor Reduced. Lane 3: ~1 ug of purified Soybean Trypsin Inhibitor Non-reduced. Lane 4: 0.25 ug of purified Soybean Trypsin Inhibitor Non-reduced. Primary antibody: Soybean Trypsin Inhibitor antibody at 1:5000 for overnight at 4C. Secondary antibody: Dylight 488 conjugated Streptavidin, 1:5K and Atto 425 conjugated goat anti rabbit, 1:10K at 1.5 hr at RT. Block overnight at 4C. Predicted/Observed size: 24 kDa, 17 kDa for Trypsin.

Western Blot: Trypsin Inhibitor Antibody [NBP1-78088] - Analysis using the Biotin conjugate of NBP1-78088. Detection of Lane 1: ~1 ug of purified Soybean Trypsin Inhibitor Reduced. Lane 2: 0.25 ug of purified Soybean Trypsin Inhibitor Reduced. Lane 3: ~1 ug of purified Soybean Trypsin Inhibitor Non-reduced. Lane 4: 0.25 ug of purified Soybean Trypsin Inhibitor Non-reduced.

• Reactivity: Pl
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: LYOPH

Trypsin Inhibitor Antibody - BSA Free Summary

• Description: This antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Trypsin Inhibitor [Lima Bean]
  
  Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
• Immunogen: Trypsin Inhibitor [Lima Bean] (Uniprot: P01070)
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: KTI3
• Purity: Multi-step

Applications/Dilutions

• Dilutions:
  • ELISA 1:5000-1:25000
  • Western Blot 1:500-1:3000
• Application Notes: This product has been assayed against 1.0 ug of Trypsin Inhibitor [Lima Bean] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:15,000 of the reconstitution concentration is suggested for this product.

Reactivity Notes

Cross reactivity against Trypsin Inhibitor from other tissues and species may occur but have not been specifically determined.

Packaging, Storage & Formulations

• Storage: Store lyophilized antibody at 4C. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Preservative: 0.01% Sodium Azide
• Concentration: LYOPH
• Purity: Multi-step
• Reconstitution Instructions: Reconstitute with 100 ul deionized water (or equivalent)

Alternate Names for Trypsin Inhibitor Antibody - BSA Free

• KTI3
• Kunitz trypsin inhibitor

Background

The soybean trypsin inhibitor was first crystallized by Kunitz in 1945 and is one of several such inhibitors found in soybeans. It inhibits the proteolytic action approximately of an equal weight of crystalline trypsin by combining with trypsin to form a stable compound. Chymotrypsin is only slightly inhibited by soy inhibitor.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: KTI3
• Uniprot:
  • P01070(Plant)