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![Western Blot: Trypsin Inhibitor Antibody [NBP2-21629] - To detect target proteins Trypsin Inhibitor (left) and Streptavidin (right) under reducing (R) and non-reducing (NR) conditions. Reduced samples of purified target proteins contained 4% BME and were boiled for 5 minutes. Samples of ~1ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with 1:1000 dilution of primary antibody](http://images.novusbio.com/fullsize/Trypsin-Inhibitor-Antibody-Western-Blot-NBP2-21629-img0003.jpg "Western Blot: Trypsin Inhibitor Antibody [NBP2-21629] - To detect target proteins Trypsin Inhibitor (left) and Streptavidin (right) under reducing (R) and non-reducing (NR) conditions. Reduced samples of purified target proteins contained 4% BME and were boiled for 5 minutes. Samples of ~1ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with 1:1000 dilution of primary antibody")
![Western Blot: Trypsin Inhibitor Antibody [NBP2-21629] - Rabbit anti Soybean Trypsin Inhibitor antibody was used to detect purified Soybean Trypsin Inhibitor. Samples of ~1 and 0.25 ug of purified Soybean Trypsin Inhibitor per lane were run by SDS-PAGE under reducing and non-reducing conditions and reduced samples of protein contained 4% BME and were boiled for 5 minutes. Protein was transferred to nitrocellulose and probed with Rabbit anti Soybean Trypsin Inhibitor antibody. Primary antibody was detected with Dylight 488 conjugated Streptavidin and Atto 425 conjugated goat anti rabbit and imaged on the VersaDoc imaging system.](http://images.novusbio.com/fullsize/Trypsin-Inhibitor-Antibody-Western-Blot-NBP2-21629-img0002.jpg)
![Western Blot: Trypsin Inhibitor Antibody [NBP1-78088] - Analysis using the Biotin conjugate of NBP1-78088. Detection of Lane 1: ~1 ug of purified Soybean Trypsin Inhibitor Reduced. Lane 2: 0.25 ug of purified Soybean Trypsin Inhibitor Reduced. Lane 3: ~1 ug of purified Soybean Trypsin Inhibitor Non-reduced. Lane 4: 0.25 ug of purified Soybean Trypsin Inhibitor Non-reduced. Primary antibody: Soybean Trypsin Inhibitor antibody at 1:5000 for overnight at 4C. Secondary antibody: Dylight 488 conjugated Streptavidin, 1:5K and Atto 425 conjugated goat anti rabbit, 1:10K at 1.5 hr at RT. Block overnight at 4C. Predicted/Observed size: 24 kDa, 17 kDa for Trypsin.](http://images.novusbio.com/fullsize/Trypsin Inhibitor Antibody-Western Blot-NBP1-78088-img0006.jpg "Western Blot: Trypsin Inhibitor Antibody [NBP1-78088] - Analysis using the Biotin conjugate of NBP1-78088. Detection of Lane 1: ~1 ug of purified Soybean Trypsin Inhibitor Reduced. Lane 2: 0.25 ug of purified Soybean Trypsin Inhibitor Reduced. Lane 3: ~1 ug of purified Soybean Trypsin Inhibitor Non-reduced. Lane 4: 0.25 ug of purified Soybean Trypsin Inhibitor Non-reduced. Primary antibody: Soybean Trypsin Inhibitor antibody at 1:5000 for overnight at 4C. Secondary antibody: Dylight 488 conjugated Streptavidin, 1:5K and Atto 425 conjugated goat anti rabbit, 1:10K at 1.5 hr at RT. Block overnight at 4C. Predicted/Observed size: 24 kDa, 17 kDa for Trypsin.")
![Western Blot: Trypsin Inhibitor Antibody [NBP1-78088] - Analysis using the Biotin conjugate of NBP1-78088. Detection of Lane 1: ~1 ug of purified Soybean Trypsin Inhibitor Reduced. Lane 2: 0.25 ug of purified Soybean Trypsin Inhibitor Reduced. Lane 3: ~1 ug of purified Soybean Trypsin Inhibitor Non-reduced. Lane 4: 0.25 ug of purified Soybean Trypsin Inhibitor Non-reduced.](http://images.novusbio.com/fullsize/Trypsin-Inhibitor-Antibody-Western-Blot-NBP1-78088-img0003.jpg)