TRA-1-85/CD147 Antibody (TRA-1-85) [Biotin]

TRA‑1‑85/CD147 was detected in immersion fixed THP‑1 human acute monocytic leukemia cell line using Mouse Anti-Human TRA‑1‑85/CD147 Biotinylated Monoclonal Antibody (Catalog # BAM3195) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surface and cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

• Reactivity: Hu
• Applications: WB, Flow, ICC/IF
• Clone: TRA-1-85
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Biotin
• Concentration: LYOPH

TRA-1-85/CD147 Antibody (TRA-1-85) [Biotin] Summary

• Immunogen: 2120Ep human embryonal carcinoma cell line
• Specificity: Detects human TRA‑1‑85.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: BSG
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 2.5 ug/10^6 cells
  • Immunocytochemistry 8-25 ug/mL
  • Western Blot
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TRA-1-85/CD147 Antibody (TRA-1-85) [Biotin]

• OKa Blood Group Antigen
• TRA185
• TRA-1-85

Background

The TRA-1-85 clone recognizes the cell surface expressed Oka blood group antigen in almost all human cell types (1). This antibody is frequently used to identify human cells.

1. Williams, B.P. et al. (1988) Immunogenetics. 27:322.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: BSG