TRA-1-85/CD147 Antibody (TRA-1-85) [Phycoerythrin]

Human peripheral blood lymphocytes and monocytes were stained with either (A) Mouse Anti-Human TRA-1-85/CD147 PE-conjugated Monoclonal Antibody (Catalog # FAB3195P) or (B) Mouse IgG1Phycoerythrin Isotype Control (Catalog # IC002P). View our protocol for Staining Membrane-associated Proteins.

Flow cytometry analysis of total cell fractions of dissociated cells from PDX models.A) Basal-like xenograft cells. B) Luminal-like xenograft cells. A and B displays pseudo-color dot plots (left panels) and histograms (right panels). Freshly harvested xenografts were minced and the whole cell suspensions were washed and stained with monoclonal antibody towards EpCAM, TRA-1-85 (filled blue in histograms), H2-kd (red line in lower histogram) and Hoecst-33342 (intensity measure for DNA content of cells, grey contours in both histograms. Left peak indicate mouse cells, right peak indicate human cells). The population positive for both EpCAM and TRA-1-85, i.e the human tumor cells, are indicated with a circle in the dot plots. C) Flow cytometry analysis of double stained samples (marker of interest and EpCAM/Tra-1-85) of the Luminal-like PDX model. Flow cytometry histograms show the distribution of the markers indicated in the figure. Filled blue histogram represents EpCAM positive tumor cell population, and the EpCAM negative population (mouse stroma cells) is indicated by the red line. Grey contour represent unstained control. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25419568), licensed under a CC-BY license. Not internally tested by R&D Systems.

Flow cytometry analysis of total cell fractions of dissociated cells from PDX models.A) Basal-like xenograft cells. B) Luminal-like xenograft cells. A and B displays pseudo-color dot plots (left panels) and histograms (right panels). Freshly harvested xenografts were minced and the whole cell suspensions were washed and stained with monoclonal antibody towards EpCAM, TRA-1-85 (filled blue in histograms), H2-kd (red line in lower histogram) and Hoecst-33342 (intensity measure for DNA content of cells, grey contours in both histograms. Left peak indicate mouse cells, right peak indicate human cells). The population positive for both EpCAM and TRA-1-85, i.e the human tumor cells, are indicated with a circle in the dot plots. C) Flow cytometry analysis of double stained samples (marker of interest and EpCAM/Tra-1-85) of the Luminal-like PDX model. Flow cytometry histograms show the distribution of the markers indicated in the figure. Filled blue histogram represents EpCAM positive tumor cell population, and the EpCAM negative population (mouse stroma cells) is indicated by the red line. Grey contour represent unstained control. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25419568), licensed under a CC-BY license. Not internally tested by R&D Systems.

Flow cytometry analysis of total cell fractions of dissociated cells from PDX models.A) Basal-like xenograft cells. B) Luminal-like xenograft cells. A and B displays pseudo-color dot plots (left panels) and histograms (right panels). Freshly harvested xenografts were minced and the whole cell suspensions were washed and stained with monoclonal antibody towards EpCAM, TRA-1-85 (filled blue in histograms), H2-kd (red line in lower histogram) and Hoecst-33342 (intensity measure for DNA content of cells, grey contours in both histograms. Left peak indicate mouse cells, right peak indicate human cells). The population positive for both EpCAM and TRA-1-85, i.e the human tumor cells, are indicated with a circle in the dot plots. C) Flow cytometry analysis of double stained samples (marker of interest and EpCAM/Tra-1-85) of the Luminal-like PDX model. Flow cytometry histograms show the distribution of the markers indicated in the figure. Filled blue histogram represents EpCAM positive tumor cell population, and the EpCAM negative population (mouse stroma cells) is indicated by the red line. Grey contour represent unstained control. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25419568), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Hu
• Applications: Flow
• Clone: TRA-1-85
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Phycoerythrin

TRA-1-85/CD147 Antibody (TRA-1-85) [Phycoerythrin] Summary

• Immunogen: 2120Ep human embryonal carcinoma cell line
• Specificity: Detects human TRA‑1‑85 antigen in flow cytometry.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: BSG
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TRA-1-85/CD147 Antibody (TRA-1-85) [Phycoerythrin]

• OKa Blood Group Antigen
• TRA185
• TRA-1-85

Background

The TRA-1-85 antigen, also known as OKa blood group antigen, is a specific epitope within the protein known as Basigin, EMMPRIN and CD147. It is a cell surface determinant expressed on almost all human cell types. This antibody has been used in somatic cell hybrid studies to identify tissues of partial human origin (1, 2).

1. Williams, B.P. et al. (1988) Immunogenetics. 27:322.
2. Spring, F.A. et al. (1997) Eur. J. Immunol. 27:891.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TRA-1-85/CD147 Antibody (FAB3195P)(7)

Publications using FAB3195P

• Fei-Man Hsu, Qiu Ya Wu, Emily B. Fabyanic, Alex Wei, Hao Wu, Amander T. Clark TET1 facilitates specification of early human lineages including germ cells iScience 2023-07-21 [PMID: 37456839] (Flow Cytometry, Human)
• EC Pandolfi, TJ Hunt, S Goldsmith, K Hurlbut, SJ Silber, AT Clark Generation of three human induced pluripotent stem cell sublines (UCLAi004-A, UCLAi004-B, and UCLAi004-C) for reproductive science research Stem Cell Research, 2021-06-24;54(0):102446. 2021-06-24 [PMID: 34216981] (ICC, Human)
• EC Pandolfi, TJ Hunt, S Goldsmith, K Hurlbut, SJ Silber, AT Clark Generation of three human induced pluripotent stem cell sublines (UCLAi005-A, UCLAi005-B and UCLAi005-C) for reproductive science research Stem Cell Research, 2021-06-08;54(0):102409. 2021-06-08 [PMID: 34130154] (IHC, Human)
• EC Pandolfi, EJ Rojas, E Sosa, JJ Gell, TJ Hunt, S Goldsmith, Y Fan, SJ Silber, AT Clark Generation of three human induced pluripotent stem cell sublines (MZT04D, MZT04J, MZT04C) for reproductive science research Stem Cell Res, 2019-09-16;40(0):101576. 2019-09-16 [PMID: 31622877] (Flow Cytometry, Human)
• Skrbo N, Hjortland G, Kristian A, Holm R, Nord S, Prasmickaite L, Engebraaten O, Maelandsmo G, Sorlie T, Andersen K Differential in vivo tumorigenicity of distinct subpopulations from a luminal-like breast cancer xenograft. PLoS ONE, 2014-11-24;9(11):e113278. 2014-11-24 [PMID: 25419568] (Flow Cytometry, Human)
• Koji Tanabe, Michiko Nakamura, Megumi Narita, Kazutoshi Takahashi, Shinya Yamanaka Maturation, not initiation, is the major roadblock during reprogramming toward pluripotency from human fibroblasts Proceedings of the National Academy of Sciences 2013-07-23 [PMID: 23812749] (Flow Cytometry, Human)
• Storm MP, Orchard CB, Bone HK, Chaudhuri JB, Welham MJ Three-dimensional culture systems for the expansion of pluripotent embryonic stem cells. Biotechnol. Bioeng., 2010-11-01;107(4):683-95. 2010-11-01 [PMID: 20589846] (Flow Cytometry, ICC, Human)

Bioinformatics

• Gene Symbol: BSG