![Immunocytochemistry/Immunofluorescence: TMEM145 Antibody [NBP2-57030] - Staining of human cell line SH-SY5Y shows localization to vesicles.](http://images.novusbio.com/fullsize/TMEM145-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-57030-img0001.jpg "Immunocytochemistry/Immunofluorescence: TMEM145 Antibody [NBP2-57030] - Staining of human cell line SH-SY5Y shows localization to vesicles.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Description | Novus Biologicals Rabbit TMEM145 Antibody - BSA Free (NBP2-57030) is a polyclonal antibody validated for use in IHC and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee. |
| Immunogen | This antibody was developed against a recombinant protein corresponding to the following amino acid sequence: MTRPSAANKNFPYHVRTSQIASAGVPGPGGSQSADKAFPQHVYGNVTFISDS |
| Predicted Species | Mouse (100%), Rat (98%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | TMEM145 |
| Purity | Affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100. |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.2) and 40% Glycerol |
| Preservative | 0.02% Sodium Azide |
| Purity | Affinity purified |
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | TMEM145 |
![Immunohistochemistry-Paraffin: TMEM145 Antibody [NBP2-57030] - Staining in human cerebral cortex and pancreas tissues using anti-TMEM145 antibody. Corresponding TMEM145 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/TMEM145-Antibody-Immunohistochemistry-Paraffin-NBP2-57030-img0006.jpg "Immunohistochemistry-Paraffin: TMEM145 Antibody [NBP2-57030] - Staining in human cerebral cortex and pancreas tissues using anti-TMEM145 antibody. Corresponding TMEM145 RNA-seq data are presented for the same tissues.")
![Immunohistochemistry-Paraffin: TMEM145 Antibody [NBP2-57030] - Immunohistochemical staining of human cerebral cortex shows weak cytoplasmic positivity in neuropil and a subset of neuronal cells.](http://images.novusbio.com/fullsize/TMEM145-Antibody-Immunohistochemistry-Paraffin-NBP2-57030-img0002.jpg "Immunohistochemistry-Paraffin: TMEM145 Antibody [NBP2-57030] - Immunohistochemical staining of human cerebral cortex shows weak cytoplasmic positivity in neuropil and a subset of neuronal cells.")
![Immunohistochemistry-Paraffin: TMEM145 Antibody [NBP2-57030] - Staining of human cerebral cortex shows strong cytoplasmic positivity in a subset of neurons.](http://images.novusbio.com/fullsize/TMEM145-Antibody-Immunohistochemistry-Paraffin-NBP2-57030-img0004.jpg "Immunohistochemistry-Paraffin: TMEM145 Antibody [NBP2-57030] - Staining of human cerebral cortex shows strong cytoplasmic positivity in a subset of neurons.")
![Immunohistochemistry-Paraffin: TMEM145 Antibody [NBP2-57030] - Staining of human pancreas shows low expression as expected.](http://images.novusbio.com/fullsize/TMEM145-Antibody-Immunohistochemistry-Paraffin-NBP2-57030-img0005.jpg "Immunohistochemistry-Paraffin: TMEM145 Antibody [NBP2-57030] - Staining of human pancreas shows low expression as expected.")
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")