TEV Protease Antibody

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Western Blot: TEV Protease Antibody [NBP1-97669] - E.coli lysate containing recombinant TEV protease, NBP1-97669 used at 1:1000 dilution.

Product Details

Summary
Reactivity V-ViSpecies Glossary
Applications WB, ELISA
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

TEV Protease Antibody Summary

Immunogen
This protein-A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with recombinant MBP-and-poly-His-tagged autoinactivation-resistant mutant TEV Protease.
Specificity
This antibody was cross-adsorbed against MBP from monospecific antiserum by chromatography. Assay by western blot showed no reactivity to MBP.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Purity
Protein A purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:500
  • ELISA 1:1500-1:27000
Application Notes
Expect a band approximately 27 kDa in size corresponding to TEV by western blotting in the appropriate cell lysate or extract. Anti-TEV protease may be useful - detect residual TEV protease in preparations of recombinant proteins in which that protease may interfere with downstream manipulations.
Publications
Read Publication using
NBP1-97669 in the following applications:

  • WB
    1 publication

Reactivity Notes

Reactive with Tobacco Etch Virus

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein A purified

Alternate Names for TEV Protease Antibody

  • NIa
  • P1 Protease
  • TEV Protease
  • Tobacco Etch Virus Protease

Background

TEV protease, encoded by the Tobacco Etch Virus (TEV), is a 27 kDa catalytic domain of the Nuclear Inclusion a (NIa) protein encoded by the virus (TEV). It is widely used for cleaving fusion proteins because of its sequence specificity. It recognizes a linear epitope of the general form E-Xaa-Xaa-Y-Xaa-Q-(G/S). Cleavage occurs between Q and G or Q and S. The structure of TEV protease is similar to that of of the serine protease family. Like serine proteases, TEV protease utilizes a catalytic triad of residues to hydrolyze peptide bonds. The distinguishing feature of TEV protease, however, is that instead of the serine nucleophile in the triad Ser-Asp-His, there is a cysteine, which may explain the resistance of TEV protease to protease inhibitors which are commonly used. The strain used is the autoinactivation-resistant mutant S219V. The catalytic activity of the S219V mutant is approximately 2 fold greater than that of the wild-type protease.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TEV Protease Antibody (NBP1-97669)(1)

We have publications tested in 1 confirmed species: Virus.

We have publications tested in 1 application: WB.


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WB
(1)
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Showing Publication 1 - 1 of 1.
Publication using NBP1-97669 Applications Species
Roderer D, Schubert E, Sitsel O, Raunser S Towards the application of Tc toxins as a universal protein translocation system bioRxiv Jul 18 2019 (WB, Virus)

Details:
Tobacco Etch Virus
WB Virus

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for TEV Protease Antibody (NBP1-97669). (Showing 1 - 1 of 1 FAQ).

  1. Our customer who purchased NBP1-97669 would like to ask if the positive control for WB can be provided? In addition, there seems no suggested positive control listed. Would you please also help suggest?
    • Purified enhanced TEV should be fine commercially available from companies such as Sigma, Genway or Invitrogen. The end-user can also test against lysates transfected with any TEV designated autoinactivation-resistant mutant S219V
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