Expect a band approximately 27 kDa in size corresponding to TEV by western blotting in the appropriate cell lysate or extract. Anti-TEV protease may be useful - detect residual TEV protease in preparations of recombinant proteins in which that protease may interfere with downstream manipulations.
Read Publication using NBP1-97669 in the following applications:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% Sodium Azide
Protein A purified
Alternate Names for TEV Protease Antibody
Tobacco Etch Virus Protease
TEV protease, encoded by the Tobacco Etch Virus (TEV), is a 27 kDa catalytic domain of the Nuclear Inclusion a (NIa) protein encoded by the virus (TEV). It is widely used for cleaving fusion proteins because of its sequence specificity. It recognizes a linear epitope of the general form E-Xaa-Xaa-Y-Xaa-Q-(G/S). Cleavage occurs between Q and G or Q and S. The structure of TEV protease is similar to that of of the serine protease family. Like serine proteases, TEV protease utilizes a catalytic triad of residues to hydrolyze peptide bonds. The distinguishing feature of TEV protease, however, is that instead of the serine nucleophile in the triad Ser-Asp-His, there is a cysteine, which may explain the resistance of TEV protease to protease inhibitors which are commonly used. The strain used is the autoinactivation-resistant mutant S219V. The catalytic activity of the S219V mutant is approximately 2 fold greater than that of the wild-type protease.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Publications for TEV Protease Antibody (NBP1-97669)(1)
We have publications tested in 1 confirmed species: Virus.
We have publications tested in 1 application: WB.
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Publication using NBP1-97669
Roderer D, Schubert E, Sitsel O, Raunser S Towards the application of Tc toxins as a universal protein translocation system bioRxiv Jul 18 2019 (WB, Virus)
Details: Tobacco Etch Virus
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Our customer who purchased NBP1-97669 would like to ask if the positive control for WB can be provided? In addition, there seems no suggested positive control listed. Would you please also help suggest?
Purified enhanced TEV should be fine commercially available from companies such as Sigma, Genway or Invitrogen. The end-user can also test against lysates transfected with any TEV designated autoinactivation-resistant mutant S219V
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