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![Western Blot: TEV Protease Antibody [NBP1-97669] - No translocation was observed for TEV protease cargo alone. Western blots show the presence of the constructs at lower retention volumes during size exclusion chromatography both at pH 8 (Left) and pH 11 (Right), meaning they co-localize with the rest of the holotoxin even after prepore-to-pore transition and are not translocated. Note: Lower retention volume towards left of blot and higher retention volume toward right. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31748551/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/TEV-Protease-Antibody-Western-Blot-NBP1-97669-img0002.jpg "Western Blot: TEV Protease Antibody [NBP1-97669] - No translocation was observed for TEV protease cargo alone. Western blots show the presence of the constructs at lower retention volumes during size exclusion chromatography both at pH 8 (Left) and pH 11 (Right), meaning they co-localize with the rest of the holotoxin even after prepore-to-pore transition and are not translocated. Note: Lower retention volume towards left of blot and higher retention volume toward right. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31748551/) licensed under a CC-BY license.")
![Western Blot: TEV Protease Antibody [NBP1-97669] - E.coli lysate containing recombinant TEV protease, NBP1-97669 used at 1:1000 dilution.](http://images.novusbio.com/fullsize/TEV-Protease-Antibody-Western-Blot-NBP1-97669-img0001.jpg)
![Western Blot: TEV Protease Antibody (9A10-4C3) [NBP2-29909] - Analysis of TEV cleavage site was performed by loading the indicated amounts of TEV control protein and 5ul of Lane Marker Reducing Sample Buffer per well onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with a TEV cleavage site monoclonal antibody at a dilution of 1:1000 overnight at 4C on a rocking platform, washed in TBS-0.1%Tween 20, and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:15,000 for at least 1 hour. Chemiluminescent detection was performed using SuperSignal West Dura.](http://images.novusbio.com/fullsize/TEV-Protease-Antibody-9A10-4C3-Western-Blot-NBP2-29909-img0003.jpg "Western Blot: TEV Protease Antibody (9A10-4C3) [NBP2-29909] - Analysis of TEV cleavage site was performed by loading the indicated amounts of TEV control protein and 5ul of Lane Marker Reducing Sample Buffer per well onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with a TEV cleavage site monoclonal antibody at a dilution of 1:1000 overnight at 4C on a rocking platform, washed in TBS-0.1%Tween 20, and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:15,000 for at least 1 hour. Chemiluminescent detection was performed using SuperSignal West Dura.")