Swi6 Antibody

Images

 
Western Blot: Swi6 Antibody [NB600-932] - Analysis of endogenous protein present in S.pombe lysate (arrowhead). Comparison to a molecular weight marker (not shown) indicates a band of 43 kDa corresponding to S.pombe ...read more
Western Blot: Swi6 Antibody [NB600-932] - Analysis is shown using Affinity Purified anti-Swi6 antibody to detect endogenous protein present in S.pombe lysate (arrowhead). Comparison to a molecular weight marker (not ...read more

Product Details

Summary
Reactivity YSpecies Glossary
Applications WB, ELISA
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

Swi6 Antibody Summary

Immunogen
This Swi6 Antibody was prepared from whole rabbit antiserum produced by repeated immunizations with a synthetic peptide corresponding aa 314-328 of S.pombe Swi6 protein. (Uniprot: P40381)
Localization
Nuclear
Specificity
Swi6
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • ELISA 1:5000-1:30000
  • Western Blot 1:500-1:3000
Application Notes
Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 37 kDa in size corresponding to Swi6 protein by western blotting in the appropriate cell lysate or extract.
Theoretical MW
37.29 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Reactive with Swi6 from Schizosaccharomyces pombe.
BLAST analysis indicates that cross reactivity with homologues of this protein from other sources is not likely

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Purity
Immunogen affinity purified

Notes

Swi6 Antibody is an affinity purified antibody produced by immunoaffinity chromatography using peptide coupled to agarose beads followed by solid phase adsorption to remove any unwanted reactivities

Store this antibody at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Alternate Names for Swi6 Antibody

  • Chromatin-associated swi6 protein; Regulatory protein SWI6

Background

The chromatin structure of Fission yeast centromeres is regulated epigenetically. In the central core domain, the histone H3 variant Cnp1(CENP-A) plays a key role. In the flanking heterochromatin domain, histones are kept underacetylated by the histone deacetylases (HDACs) Clr3, Clr6 and Sir2, and methylated by Clr4 methyltransferase (HMTase) to create a specific binding site for the Swi6 protein. Swi6 then directly mediates cohesin binding to the centromeric heterochromatin. Recognizes and binds histone H3 tails methylated at Lys-9, leading to epigenetic repression. Involved in the repression of the silent mating-type loci MAT2 and MAT3. May compact MAT2/3 into a heterochromatin-like conformation which represses the transcription of these silent cassettes. Swi6 interacts with histone H3 methylated at Lys-9 and with the cohesin subunit psc3. Swi6 associates with Swi4 to activate HO and many other late G1-specific transcripts in budding yeast. In contrast, histone H3 methylated at Lys9 and its interacting protein Swi6/HP1, which define heterochromatin, coated extended domains associated with a variety of repeat elements and small islands corresponding to meiotic genes. Contains two chromo domains.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

Additional Swi6 Products

Array NB600-932

Research Areas for Swi6 Antibody (NB600-932)

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