SPRYD5 Antibody - BSA Free

Western Blot: SPRYD5 Antibody [NBP1-77087] - Rat brain tissue lysate with SPRYD5 antibody at (A) 0.25 and (B) 0.5 ug/mL .

Immunohistochemistry: SPRYD5 Antibody - BSA Free [NBP1-77087] - Immunohistochemistry of SPRYD5 in mouse brain tissue with SPRYD5 antibody at 5 u/mL.

Immunocytochemistry/ Immunofluorescence: SPRYD5 Antibody - BSA Free [NBP1-77087] - Immunofluorescence of SPRYD5 in mouse brain tissue with SPRYD5 antibody at 20 ug/mL.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

SPRYD5 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit SPRYD5 Antibody - BSA Free (NBP1-77087) is a polyclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human SPRYD5. The immunogen is located within amino acids 350 - 400 of SPRYD5. Amino Acid Squence: NNYWKEKRQNDKIDGEE
• Specificity: SPRYD5 antibody is predicted to not cross-react with other SPRYD protein family members. At least two isoforms of SPRYD5 are known to exist; this antibody will detect both isoforms
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TRIM51
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 20 ug/ml
  • Immunohistochemistry 5 ug/ml
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Western Blot 0.25-0.5 ug/ml
• Control Peptide:

  SPRYD5 Peptide (NBP1-77087PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for SPRYD5 Antibody - BSA Free

• MGC10977
• SPRY domain containing 5
• SPRY domain-containing protein 5
• TRIM51
• tripartite motif-containing 51
• Tripartite motif-containing protein 51

Background

The SPRY domain-containing protein 5 (SPRYD5) is a member of a family of proteins whose sole common characteristic is the presence of a SPRY domain. SPRY domains are structural domains that were first described in the fungal Dictyostelium discoideum tyrosine kinase spore lysis A. In most systems SPRY domains provide binding sites for regulatory proteins or intramolecular binding sites that maintain the structural integrity of a protein. SPRYD5 belongs to the TRIM/RBCC family and contains one B box-type zinc finger, one B30.2/SPRY domain and one RING-type zinc finger. Little is known of the function of the SPRYD5 protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TRIM51
• Uniprot:
  • NP_116070(Human)