SPATA20 Overexpression Lysate (Native) Summary
The lysate was created in HEK293T cells, using plasmid ID RC206825 and based on accession number NM_022827. The protein contains a C-terminal DDK tag.
Homo sapiens spermatogenesis associated 20 (SPATA20), mRNA.
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Packaging, Storage & Formulations
Store at -80C. Avoid freeze-thaw cycles.
Lysate Details for SPATA20
HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.
Alternate Names for SPATA20 Overexpression Lysate (Native)
- sperm protein SSP411
- spermatogenesis associated 20
- spermatogenesis-associated protein 20
- Sperm-specific protein 411
- transcript increased in spermiogenesis 78
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed
for 6 months from date of receipt.
Customers Who Viewed This Item Also Viewed...
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P, KD
Species: Hu, Mu
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P
Species: Hu, Mu, Rt, Ha, Pm
Applications: WB, Simple Western, Flow, ICC/IF, IHC, IHC-P
Publications for SPATA20 Lysate (NBL1-16379) (0)
There are no publications for SPATA20 Lysate (NBL1-16379).
By submitting your publication information earn gift cards and discounts for future purchases.
Reviews for SPATA20 Lysate (NBL1-16379) (0)
There are no reviews for SPATA20 Lysate (NBL1-16379).
By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
- Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
- Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
- 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen
Product General Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
FAQs for SPATA20 Lysate (NBL1-16379) (0)
Additional SPATA20 Products
Bioinformatics Tool for SPATA20 Lysate (NBL1-16379)
Discover related pathways, diseases and genes to SPATA20 Lysate (NBL1-16379). Need help?
Read the Bioinformatics Tool Guide
for instructions on using this tool.
Blogs on SPATA20