SPATA20 Overexpression Lysate (Native)


Western Blot: SPATA20 Overexpression Lysate (Adult Normal) [NBL1-16379] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for SPATA20.

Product Details

Applications WB
The exact concentration of the protein of interest cannot be determined for overexpression lysates. Please contact technical support for more information.

Order Details

SPATA20 Overexpression Lysate (Native) Summary

The lysate was created in HEK293T cells, using plasmid ID RC206825 and based on accession number NM_022827. The protein contains a C-terminal DDK tag.
Homo sapiens spermatogenesis associated 20 (SPATA20), mRNA.

Packaging, Storage & Formulations

Store at -80C. Avoid freeze-thaw cycles.
RIPA buffer


  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
89.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Lysate Details for SPATA20

Protein State


HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for SPATA20 Overexpression Lysate (Native)

  • DKFZp686H1839
  • FLJ21347
  • FLJ21969
  • MGC111032
  • sperm protein SSP411
  • spermatogenesis associated 20
  • spermatogenesis-associated protein 20
  • Sperm-specific protein 411
  • SSP411
  • Tisp78
  • transcript increased in spermiogenesis 78


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu, Mu
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P
Species: Hu, Mu, Rt, Ha, Pm
Applications: WB, Simple Western, Flow, ICC/IF, IHC, IHC-P
Applications: WB

Publications for SPATA20 Lysate (NBL1-16379) (0)

There are no publications for SPATA20 Lysate (NBL1-16379).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for SPATA20 Lysate (NBL1-16379) (0)

There are no reviews for SPATA20 Lysate (NBL1-16379). By submitting a review earn points towards our Rewards Program.
  • 250 points for product review
  • 500 additional points for an image with your product review
  • Double points (500) if you are the first to review this product
  • Double points (1000) if you are the first to review this product with an image

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for SPATA20 Lysate (NBL1-16379) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional SPATA20 Overexpression Lysate (Native) Products

Related Products by Gene

Bioinformatics Tool for SPATA20 Lysate (NBL1-16379)

Discover related pathways, diseases and genes to SPATA20 Lysate (NBL1-16379). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for SPATA20 Lysate (NBL1-16379)

Discover more about diseases related to SPATA20 Lysate (NBL1-16379).

Pathways for SPATA20 Lysate (NBL1-16379)

View related products by pathway.

Blogs on SPATA20

There are no specific blogs for SPATA20, but you can read our latest blog posts.

Contact Information

Product PDFs


Gene Symbol SPATA20

Customer Resources

Novus Review - Submit your review and earn rewards points which can be used for merchandise & discounts.
Risk Free Testing - Test on a species/application not listed above to receive a full credit towards a future purchase.

Novus' Quality Guarantee - Novus guarantees that every product we sell will work in the application and species listed on our website and datasheets.

Submit your question on NBL1-16379 below.
During business hours, we will respond to your email within 24 hours. For any questions submitted on the weekend, a response will be received on Monday.
For immediate assistance during business hours M- F (excluding major holidays), please contact us.
Ask a Scientist - We have a lab full of white coats just waiting for your scientific questions and concerns.

Customers Who Bought This Also Bought

HSP60 Antibody
HSP60 Antibody
HSP60 Antibody
SPATA20 Antibody
SPATA20 Antibody
SPATA20 Antibody
PGAM1 Antibody
PGAM1 Antibody
PGAM1 Antibody