SDCCAG10 Antibody (OTI4C8) - Azide and BSA Free

Western Blot: SDCCAG10 Antibody (OTI4C8) - Azide and BSA Free [NBP2-74031] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SDCCAG10.

Immunohistochemistry: SDCCAG10 Antibody (OTI4C8) - Azide and BSA Free [NBP2-74031] - Analysis of Human thyroid tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)

Immunohistochemistry: SDCCAG10 Antibody (OTI4C8) - Azide and BSA Free [NBP2-74031] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)

Immunohistochemistry: SDCCAG10 Antibody (OTI4C8) - Azide and BSA Free [NBP2-74031] - Analysis of Carcinoma of Human pancreas tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)

Immunohistochemistry: SDCCAG10 Antibody (OTI4C8) - Azide and BSA Free [NBP2-74031] - Analysis of Human colon tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)

Immunohistochemistry: SDCCAG10 Antibody (OTI4C8) - Azide and BSA Free [NBP2-74031] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)

Immunohistochemistry: SDCCAG10 Antibody (OTI4C8) - Azide and BSA Free [NBP2-74031] - Analysis of Human liver tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)

Immunohistochemistry: SDCCAG10 Antibody (OTI4C8) - Azide and BSA Free [NBP2-74031] - Analysis of Human lymphoma tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)

Immunohistochemistry: SDCCAG10 Antibody (OTI4C8) - Azide and BSA Free [NBP2-74031] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)

Immunohistochemistry: SDCCAG10 Antibody (OTI4C8) - Azide and BSA Free [NBP2-74031] - Analysis of Human prostate tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)

Immunohistochemistry: SDCCAG10 Antibody (OTI4C8) - Azide and BSA Free [NBP2-74031] - Analysis of Human tonsil tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clone: OTI4C8
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

SDCCAG10 Antibody (OTI4C8) - Azide and BSA Free Summary

• Immunogen: Human recombinant protein fragment corresponding to amino acids 145-472 of human CWC27(NP_005860) produced in E.coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CWC27
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Western Blot 1:2000
• Theoretical MW: 53.7 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for SDCCAG10 Antibody (OTI4C8) - Azide and BSA Free

• Antigen NY-CO-10
• CWC27 spliceosome-associated protein homolog (S. cerevisiae)
• EC 5.2.1.8
• NY-CO-10
• peptidyl-prolyl cis-trans isomerase SDCCAG10
• PPIase CWC27
• PPIase SDCCAG10
• SDCCAG10
• Serologically defined colon cancer antigen 10peptidyl-prolyl cis-trans isomerase CWC27 homolog

Background

PPIases accelerate the folding of proteins

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CWC27