SARS Spike Protein Antibody (16F1071) - BSA Free

• Reactivity: V
• Applications: WB, ICC/IF
• Clone: 16F1071
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

SARS Spike Protein Antibody (16F1071) - BSA Free Summary

• Description: Novus Biologicals Mouse SARS Spike Protein Antibody (16F1071) - BSA Free (NBP2-24746) is a monoclonal antibody validated for use in WB and ICC/IF. Anti-SARS Spike Protein Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The antibody was developed by immunizing mice with a synthetic peptide corresponding to amino acids 19-35 (CTTFDDVQAPNYTQHTS) from the S (Spike glycoprotein)(Spike Protein 1) for the Human SARS coronavirus (Genbank accession no. NP_828851.1)
• Preparation Method: The C1.18.4 hybridoma cell line was grown and tissue culture supernatant was purified using affinity chromatography.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence reported in scientific literature (PMID 16081529)
  • Western Blot 1-3 ug/ml

Reactivity Notes

This antibody is not reactive to COVID-19

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for SARS Spike Protein Antibody (16F1071) - BSA Free

• E2 glycoprotein
• E2
• Human coronavirus spike glycoprotein
• Peplomer protein
• S glycoprotein
• S
• SARS coronavirus S protein
• SARS coronavirus Spike Protein
• SARS CoV S glycoprotein
• SARS CoV Spike protein
• SARS CoV
• SARS-CoV S protein
• SARS-COV Spike
• SARSCoV
• Severe acute respiratory syndrome spike glycoprotein
• Severe acute respiratory syndrome virus spike glycoprotein
• Spike glycoprotein
• VGL2

Background

It has recently been shown that SARS (severe acute respiratory syndrome) is caused by a human coronavirus. Human coronaviruses are the major cause of upper respiratory tract illness, such as the common cold, in humans. Coronaviruses are positive-stranded RNA viruses, featuring the largest viral RNA genomes known to date (27-31 kb). The first step in coronavirus infection is binding of the viral spike protein, a 139-kDa protein, to certain receptors on host cells. The spike protein is the main surface antigen of the coronavirus. The glycosilated spike protein (as well as the nucleocapsid protein) can be detected in infected cell culture supernatants with antisera from SARS patients.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SARS Spike Protein Antibody (NBP2-24746)(2)

Publications using NBP2-24746

• Zhang L, Wei L, Jiang D et al. SARS-CoV nucleocapsid protein induced apoptosis of COS-1 mediated by the mitochondrial pathway. Artif Cells Blood Substit Immobil Biotechnol. 2007-01-01 [PMID: 17453707]
  
  Details:
  WB: Fig. 1 (COS-1, Huh-7, and Hep2 transiently or mock-transfected with SARS Matrix, Spike, or Nucleocapsid proteins), Antibodies were transfected validated. Imgenex products cited: 1. SARS-Spike (IMG-5119A) 2. SARS-Spike (IMG-5015) 3. SARS-M (IMG-5125A).
• Simmons G, Gosalia DN, Rennekamp AJ et al. Inhibitors of cathepsin L prevent severe acute respiratory syndrome coronavirus entry. Proc Natl Acad Sci U S A. 2005-08-16 [PMID: 16081529] (ICC/IF)

FAQs for SARS Spike Protein Antibody (NBP2-24746). (Showing 1 - 2 of 2 FAQs).

1. Do you know if Sars Spike Protein Antibody (16F1071) will cross react with SARS-2?
   • NBP2-24746 has no similarity unfortunately. However, NBP2-24808, NBP2-24942, and NB100-56578 have 100% sequence homology with the SARS-CoV-2 Spike Protein.
2. I am looking for a primary antibody to sars spike protein to the new COVID-19.
   • NBP1-28850, NB100-56578, NBP2-24808, NBP2-24942 have 100% homology to the new SARS-CoV-2 virus.