SARS Nucleocapsid Protein Antibody - BSA Free

Western Blot: SARS Nucleocapsid Protein Antibody [NB100-56050] - Analysis of SARS Nucleocapsid in (A) transfected mouse melanoma cell lysate and (B) untransfected cell lysate using this antibody.

• Reactivity: V
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 0.5 mg/ml

SARS Nucleocapsid Protein Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit SARS Nucleocapsid Protein Antibody - BSA Free (NB100-56050) is a polyclonal antibody validated for use in WB and ELISA. Anti-SARS Nucleocapsid Protein Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The antibody was developed by immunizing rabbits with a synthetic peptide corresponding to amino acids 20-34 (PSDSTGSNQNGERSG) from the N (SARS Nucleocapsid) for the Human SARS coronavirus (Genbank accession no. YP_009724397.2)
• Specificity: The was tested on a human cell line transfected with full-length SARS Nucleocapsid cDNA with a predicted molecular weight of 46 kDa.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: N
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Western Blot 1:100-1:2000

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: COVID-19 (71%)

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for SARS Nucleocapsid Protein Antibody - BSA Free

• N protein
• N structural protein
• N
• NC
• Nucleocapsid protein
• Nucleoprotein
• Protein N
• SARS coronavirus N protein
• SARS coronavirus nucleocapsid protein
• SARS CoV N protein
• SARS CoV nucleocapsid protein
• SARS CoV
• SARS N protein
• SARS Nucleoprotein
• SARSCoV N protein
• SARSCoV nucleocapsid protein
• SARSCoV
• Severe acute respiratory syndrome

Background

It has recently been shown that SARS is caused by a human coronavirus. Human coronaviruses are the major cause of upper respiratory tract illness in humans, such as the common cold. Coronaviruses are positive-stranded RNA viruses, featuring the largest viral RNA genomes known to date (27-31 kb). The first step in coronavirus infection is binding of the viral spike protein, a 139-kDa protein, to certain receptors on host cells. The spike protein is the main surface antigen of the coronavirus. The most prominent protein in the culture supernatants infected with SARS virus is a 46 kDa nucleocapsid protein. This suggests that the nucleocapsid protein is a major immunogen that may be useful for early diagnostics.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SARS Nucleocapsid Protein Antibody (NB100-56050)(1)

Publication using NB100-56050

• Zhang L, Wei L, Jiang D et al. SARS-CoV nucleocapsid protein induced apoptosis of COS-1 mediated by the mitochondrial pathway. Artif Cells Blood Substit Immobil Biotechnol. 2007-01-01 [PMID: 17453707]
  
  Details:
  WB: Fig. 1 (COS-1, Huh-7, and Hep2 transiently or mock-transfected with SARS Matrix, Spike, or Nucleocapsid proteins), Antibodies were transfected validated. Imgenex products cited: 1. SARS-Spike (IMG-5119A) 2. SARS-Spike (IMG-5015) 3. SARS-M (IMG-5125A).

Bioinformatics

• Gene Symbol: N