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SARS Nucleocapsid Protein Antibody

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Western Blot: SARS Nucleocapsid Protein Antibody [NB100-56049] - Analysis of SARS Nucleocapsid in (A) transfected mouse melanoma cell lysate and (B) untransfected cell lysate using this antibody.

Product Details

Summary
Reactivity V, VSpecies Glossary
Applications WB, ELISA
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

SARS Nucleocapsid Protein Antibody Summary

Immunogen
The antibody was developed by immunizing Rabbit with a synthetic peptide corresponding to amino acids 84-99 (C-IGYYRRATRRIRGGDG) from the N (SARS Nucleocapsid) for the Human SARS coronavirus (Genbank accession no. YP_009724397.2)
Predicted Species
SARS-CoV-2 (94%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
N
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1:100-1:2000
  • Western Blot 1:100-1:2000
Application Notes
The antibody was tested on a cell line transfected with full-length SARS Nucleocapsid cDNA with a predicted molecular weight of 46 kDa.
Publications
Read Publications using NB100-56049.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: COVID-19 (94%)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein A purified

Alternate Names for SARS Nucleocapsid Protein Antibody

  • COVID-19 nucleocapsid protein
  • N protein
  • N structural protein
  • N
  • NC
  • Nucleocapsid protein
  • Nucleoprotein
  • Protein N
  • SARS coronavirus N protein
  • SARS coronavirus nucleocapsid protein
  • SARS CoV N protein
  • SARS CoV nucleocapsid protein
  • SARS CoV
  • SARS N protein
  • SARS Nucleoprotein
  • SARSCoV N protein
  • SARSCoV nucleocapsid protein
  • SARSCoV
  • SARSCOV2 N protein
  • SARS-COV-2 nucleocapsid protein
  • Severe acute respiratory syndrome

Background

It has recently been shown that SARS is caused by a human coronavirus. Human coronaviruses are the major cause of upper respiratory tract illness in humans, such as the common cold. Coronaviruses are positive-stranded RNA viruses, featuring the largest viral RNA genomes known to date (27-31 kb). The first step in coronavirus infection is binding of the viral spike protein, a 139-kDa protein, to certain receptors on host cells. The spike protein is the main surface antigen of the coronavirus. The most prominent protein in the culture supernatants infected with SARS virus is a 46 kDa nucleocapsid protein. This suggests that the nucleocapsid protein is a major immunogen that may be useful for early diagnostics.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for SARS Nucleocapsid Protein Antibody (NB100-56049) (0)

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Bioinformatics

Gene Symbol N
Uniprot