Sars Membrane Protein Antibody

Western Blot: Sars Membrane Protein Antibody [NB100-56569] - Western blot analysis of Sars Membrane Protein in (A) recombinant fusion protein containing amino acids 195-210 and (B) fusion partner without these amino acids, using NB100-56569 at 0.5 ug/ml.

Immunocytochemistry/Immunofluorescence: Sars Membrane Protein Antibody [NB100-56569] - Vero cells infected with SARS-CoV-2. Blue: DAPI; Green: SARS-CoV-2 membrane protein (NB100-56569); Magenta: SARS-CoV-2 nuclecapsid protein. Image from verified customer review.

• Reactivity: V, V
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

Sars Membrane Protein Antibody Summary

• Immunogen: The antibody was developed by immunizing rabbits with a synthetic peptide corresponding to amino acids 195-210 (YSRYRIGNYKLNTDH) from the M (Membrane protein) for the Human SARS coronavirus (Genbank accession no. YP_009724393.1)
• Specificity: The NCBI accession number of the SARS matrix protein is NP_828855.1.
• Predicted Species: SARS-CoV-2 (93%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence
  • Western Blot 0.5-2 ug/ml
• Application Notes: Sars Membrane Protein Antibody validated for Immunocytochemistry/Immunofluorescence from a verified customer review.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: COVID-19 (93%)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS containing 0.2% gelatin
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for Sars Membrane Protein Antibody

• E1 glycoprotein
• Matrix glycoprotein
• Membrane glycoprotein
• SARS coronavirus M protein
• SARS coronavirus Membrane protein
• SARS CoV M glycoprotein
• SARS CoV Matrix protein
• SARS CoV
• Sars M
• Sars Matrix Protein
• SARS-CoV M protein
• SARS-CoV Membrane protein
• SARSCoV
• Severe acute respiratory syndrome

Background

A novel coronavirus has recently been identified as the causative agent of SARS (Severe Acute Respiratory Syndrome). Coronaviruses are a major cause of upper respiratory diseases in humans. The genomes of these viruses are positive-stranded RNA approximately 27-31kb in length. The M protein (Membrane protein, Matrix protein) is one of the major structural viral proteins. It is an integral membrane protein involved in the budding of the viral particles and interacts with S (Spike) protein and the nucleocapsid protein. Coronaviruses have four important viral genes with different structural proteins: a spike glycoprotein (S), a small envelope protein (E), a matrix glycoprotein (M), and a nucleocapsid protein (N).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Sars Membrane Protein Antibody (NB100-56569)(3)

Publications using NB100-56569

• Benlarbi M, Laroche G, Fink C et al. Identification and differential usage of a host metalloproteinase entry pathway by SARS-CoV-2 Delta and Omicron iScience 2022-11-18 [PMID: 36254158]
• Sullivan E, Sung P, Wu W et al. SARS-CoV-2 Virus-like Particles Produced by a Single Recombinant Baculovirus Generate Anti-S Antibody and Protect against Variant Challenge Viruses 2022-04-27 [PMID: 35632656] (WB)
• Zhang L, Wei L, Jiang D et al. SARS-CoV nucleocapsid protein induced apoptosis of COS-1 mediated by the mitochondrial pathway. Artif Cells Blood Substit Immobil Biotechnol. 2007-01-01 [PMID: 17453707]
  
  Details:
  WB: Fig. 1 (COS-1, Huh-7, and Hep2 transiently or mock-transfected with SARS Matrix, Spike, or Nucleocapsid proteins), Antibodies were transfected validated. Imgenex products cited: 1. SARS-Spike (IMG-5119A) 2. SARS-Spike (IMG-5015) 3. SARS-M (IMG-5125A).

Review for Sars Membrane Protein Antibody (NB100-56569) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Monkey.

reviewed by: Verified Customer ICC Monkey 02/04/2022

Summary

• Application: Immunocytochemistry
• Sample Tested: Vero cell
• Species: Monkey
• Lot: IN021405D-4

Comments

• Comments: ***Novus Innovators Program - new species or application used on a primary antibody.***

FAQs for Sars Membrane Protein Antibody (NB100-56569). (Showing 1 - 1 of 1 FAQs).

1. Has it been tested whether the two antobodies anti-M protein from SARS-CoV cross-react with that of SARS-CoV-2? The references are 56569SS and NBP1-28852.
   • Sars Membrane Protein Antibody NB100-56569 expected cross reactivity with SARS-CoV-2 based on immunogen sequence homology: 93%. Sars Membrane Protein Antibody (2H2C4)NBP1-28852 expected cross reactivity with SARS-CoV-2 based on immunogen sequence homology: 88%

Bioinformatics

• Entrez:
  • AFR58746.1(Virus)
• Uniprot:
  • P59596(Virus)