![ELISA: SARS-CoV-2 Spike Antibody (7C11H11) - Alpha Variant, B.1.1.7, UK [NBP3-11942] - Validation of Alpha Variant Spike Antibody with Spike S1 Protein of SARS-CoV-2 Variants. Coating Antigen: SARS-CoV-2 spike S1 proteins WT, alpha variant (B.1.1.7), beta variant (B.1.351), gamma variant (P.1), delta variant (B.1.617.2), and omicron variant (B.1.1.529), 1 ug/mL, incubate at 4C overnight. Detection Antibody: SARS-CoV-2 Spike Antibody alpha variant (B.1.1.7) UK NBP3-11942, dilution: 1000 ng/mL, incubate at RT for 1 hr. Secondary Antibody: Goat anti-mouse HRP at 1:5,000, incubate at RT for 1 hr. NBP3-11942 can specifically detect omicron and alpha variant spike S1 protein, but not spike S1 protein of WT and other tested variants.](http://images.novusbio.com/fullsize2/SARS-CoV-2-Spike-Antibody-(7C11H11)---Alpha-Variant--B.1.1.7--UK-ELISA-NBP3-11942-img0004.jpg "ELISA: SARS-CoV-2 Spike Antibody (7C11H11) - Alpha Variant, B.1.1.7, UK [NBP3-11942] - Validation of Alpha Variant Spike Antibody with Spike S1 Protein of SARS-CoV-2 Variants. Coating Antigen: SARS-CoV-2 spike S1 proteins WT, alpha variant (B.1.1.7), beta variant (B.1.351), gamma variant (P.1), delta variant (B.1.617.2), and omicron variant (B.1.1.529), 1 ug/mL, incubate at 4C overnight. Detection Antibody: SARS-CoV-2 Spike Antibody alpha variant (B.1.1.7) UK NBP3-11942, dilution: 1000 ng/mL, incubate at RT for 1 hr. Secondary Antibody: Goat anti-mouse HRP at 1:5,000, incubate at RT for 1 hr. NBP3-11942 can specifically detect omicron and alpha variant spike S1 protein, but not spike S1 protein of WT and other tested variants.")
| Reactivity | VSpecies Glossary |
| Applications | WB, ELISA |
| Clone | 7C11H11 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1 mg/ml |
| Immunogen | Antibody was raised against a peptide corresponding to 12 amino acids near the center of SARS-CoV-2 Alpha variant (B.1.1.7) Spike protein. The immunogen is located within 650-700 amino acids of SARS-CoV-2 Alpha Variant (B.1.1.7, UK) Spike protein. |
| Specificity | May cross-react with several virus of interest (VOI) variant lineages that contains P681H mutation, including B.11.318, B.1.621, B.1.621.1, P.3. But all of these lineages are rarely present in current pandemic. |
| Isotype | IgG2b Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | S |
| Purity | Peptide affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Antibody can specifically detect SARS-CoV-2 Alpha Variant (B.1.1.7, UK) S1 protein, but not SARS-CoV-2 WT Spike S1 protein by ELISA and WB. It can also detect mutant peptide, but not WT peptide. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | 0.02% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Peptide affinity purified |
Secondary Antibodies |
Isotype Controls |
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| Gene Symbol | S |
![Western Blot: SARS-CoV-2 Spike Antibody (7C11H11) - Alpha Variant, B.1.1.7, UK [NBP3-11942] - WB Validation of Alpha Variant Spike Antibodies with SARS-CoV-2 Alpha Variant Spike S1 Protein Loading: 50 ng of SARS-CoV-2 spike S1 proteins, including WT and alpha variant (B.1.1.7, UK). Detection Antibodies: SARS-CoV-2 Spike antibody, NBP3-11942, 1 ug/mL, incubate at RT for 1 hr. Secondary Antibodies: Goat anti-mouse HRP at 1:5,000, incubate at RT for 1 hr. SARS-CoV-2 Spike antibody (NBP3-11942) can specifically detect alpha variant spike S1 protein, but not WT spike S1 protein by WB.](http://images.novusbio.com/fullsize2/SARS-CoV-2-Spike-Antibody-(7C11H11)---Alpha-Variant--B.1.1.7--UK-Western-Blot-NBP3-11942-img0003.jpg "Western Blot: SARS-CoV-2 Spike Antibody (7C11H11) - Alpha Variant, B.1.1.7, UK [NBP3-11942] - WB Validation of Alpha Variant Spike Antibodies with SARS-CoV-2 Alpha Variant Spike S1 Protein Loading: 50 ng of SARS-CoV-2 spike S1 proteins, including WT and alpha variant (B.1.1.7, UK). Detection Antibodies: SARS-CoV-2 Spike antibody, NBP3-11942, 1 ug/mL, incubate at RT for 1 hr. Secondary Antibodies: Goat anti-mouse HRP at 1:5,000, incubate at RT for 1 hr. SARS-CoV-2 Spike antibody (NBP3-11942) can specifically detect alpha variant spike S1 protein, but not WT spike S1 protein by WB.")
![ELISA: SARS-CoV-2 Spike Antibody (7C11H11) - Alpha Variant, B.1.1.7, UK [NBP3-11942] - ELISA Validation of Alpha Variant Spike Antibodies with SARS-CoV-2 Alpha Variant Spike S1 Protein. Coating Antigen: SARS-CoV-2 spike S1 proteins, including WT and alpha variant (B.1.1.7, UK), 1 ug/mL, incubate at 4C overnight. Detection Antibodies: SARS-CoV-2 Spike antibody, NBP3-11942, dilution: 0.3-1000 ng/mL, incubate at RT for 1 hr. Secondary Antibodies: Goat anti-mouse HRP at 1:5,000, incubate at RT for 1 hr. SARS-CoV-2 Spike antibody (NBP3-11942) can specifically detect alpha variant spike S1 protein, but not WT spike S1 protein by ELISA.](http://images.novusbio.com/fullsize2/SARS-CoV-2-Spike-Antibody-(7C11H11)---Alpha-Variant--B.1.1.7--UK-ELISA-NBP3-11942-img0001.jpg "ELISA: SARS-CoV-2 Spike Antibody (7C11H11) - Alpha Variant, B.1.1.7, UK [NBP3-11942] - ELISA Validation of Alpha Variant Spike Antibodies with SARS-CoV-2 Alpha Variant Spike S1 Protein. Coating Antigen: SARS-CoV-2 spike S1 proteins, including WT and alpha variant (B.1.1.7, UK), 1 ug/mL, incubate at 4C overnight. Detection Antibodies: SARS-CoV-2 Spike antibody, NBP3-11942, dilution: 0.3-1000 ng/mL, incubate at RT for 1 hr. Secondary Antibodies: Goat anti-mouse HRP at 1:5,000, incubate at RT for 1 hr. SARS-CoV-2 Spike antibody (NBP3-11942) can specifically detect alpha variant spike S1 protein, but not WT spike S1 protein by ELISA.")
![ELISA: SARS-CoV-2 Spike Antibody (7C11H11) - Alpha Variant, B.1.1.7, UK [NBP3-11942] - ELISA Validation of Alpha Variant Spike Antibodies with Mutant and WT Peptide. Coating Antigen: SARS-CoV-2 spike S1 peptides, including WT and alpha variant (B.1.1.7, UK), 1 ug/mL, incubate at 4C overnight. Detection Antibodies: SARS-CoV-2 Spike antibody, NBP3-11942, dilution: 0.1-1000 ng/mL, incubate at RT for 1 hr. Secondary Antibodies: Goat anti-mouse HRP at 1:5,000, incubate at RT for 1 hr. SARS-CoV-2 Spike antibody (NBP3-11942) can specifically detect alpha variant spike S1 peptide (681H), but not WT peptide (681P).](http://images.novusbio.com/fullsize2/SARS-CoV-2-Spike-Antibody-(7C11H11)---Alpha-Variant--B.1.1.7--UK-ELISA-NBP3-11942-img0002.jpg "ELISA: SARS-CoV-2 Spike Antibody (7C11H11) - Alpha Variant, B.1.1.7, UK [NBP3-11942] - ELISA Validation of Alpha Variant Spike Antibodies with Mutant and WT Peptide. Coating Antigen: SARS-CoV-2 spike S1 peptides, including WT and alpha variant (B.1.1.7, UK), 1 ug/mL, incubate at 4C overnight. Detection Antibodies: SARS-CoV-2 Spike antibody, NBP3-11942, dilution: 0.1-1000 ng/mL, incubate at RT for 1 hr. Secondary Antibodies: Goat anti-mouse HRP at 1:5,000, incubate at RT for 1 hr. SARS-CoV-2 Spike antibody (NBP3-11942) can specifically detect alpha variant spike S1 peptide (681H), but not WT peptide (681P).")
