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SARS-CoV-2 nsp8 Antibody - BSA Free

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Western Blot: SARS-CoV-2 nsp8 Antibody [NBP3-07047] - Western Blot Validation with SARS-CoV-2 nsp8 Protein. Loading: 30 ng per lane of SARS-CoV-2 nsp8 protein. Antibodies: SARS-CoV-2 nsp8 (NBP3-07047), 1h incubation at ...read more
ELISA: SARS-CoV-2 nsp8 Antibody [NBP3-07047] - ELISA Validation with SARS-CoV-2 nsp8 Protein. Antibodies: SARS-CoV-2 nsp8 Antibody (NBP3-07047). A direct ELISA was performed using SARS-CoV-2 nsp8 recombinant protein as ...read more

Product Details

Summary
Reactivity V, VSpecies Glossary
Applications WB, ELISA
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

SARS-CoV-2 nsp8 Antibody - BSA Free Summary

Immunogen
This antibody was raised against a peptide corresponding to 14 amino acids near the center of SARS-CoV-2 (COVID-19) NSP8 protein. The immunogen is located within 40-90 amino acids of SARS-CoV-2 (COVID-19) NSP8.
Predicted Species
SARS-CoV (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ORF1ab
Purity
Peptide affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Western Blot 0.5 ug/ml
Application Notes
This antibody can detect 2 ng of free peptide at 1 ug/ml in ELISA.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Peptide affinity purified

Alternate Names for SARS-CoV-2 nsp8 Antibody - BSA Free

  • Non-structural protein 8
  • nsp8 replicase

Background

SARS-CoV-2 Nonstructural Protein 8 (NSP8) is one of the sixteen nonstructural proteins of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), the causative agent of COVID-19 (1). SARS-CoV-2 NSP8 is 198 amino acids (aa) with a theoretical molecular weight of 21.9 kDa (1,2). The amino acid sequence alignment of SARS-CoV and SARS-CoV-2's NSP8 has 97.5% sequence identity and 100% sequence similarity (1). SARS-CoV-2 NSP8 heterodimerizes with NSP7, which forms a complex with NSP12 (1-4). NSP8 alone in its monomeric form can also complex with NSP12, generating the RNA polymerase complex (1,4). Additionally, NSP8 is shown to interact with the SARS-CoV-2 open reading frame 6 (ORF6), encouraging RNA polymerase activity (1).

References

1. Yoshimoto F. K. (2020). The Proteins of Severe Acute Respiratory Syndrome Coronavirus-2 (SARS CoV-2 or n-COV19), the Cause of COVID-19. The Protein Journal. https://doi.org/10.1007/s10930-020-09901-4

2. Gordon, D. E., Jang, G. M., Bouhaddou, M., Xu, J., Obernier, K., White, K. M., O'Meara, M. J., Rezelj, V. V., Guo, J. Z., Swaney, D. L., Tummino, T. A., Huttenhain, R., Kaake, R. M., Richards, A. L., Tutuncuoglu, B., Foussard, H., Batra, J., Haas, K., Modak, M., Kim, M., ... Krogan, N. J. (2020). A SARS-CoV-2 protein interaction map reveals targets for drug repurposing. Nature. https://doi.org/10.1038/s41586-020-2286-9

3. Qiu, Y., & Xu, K. (2020). Functional studies of the coronavirus nonstructural proteins. STEMedicine. https://doi.org/10.37175/stemedicine.v1i2.39

4. Peng, Q., Peng, R., Yuan, B., Zhao, J., Wang, M., Wang, X., Wang, Q., Sun, Y., Fan, Z., Qi, J., Gao, G. F., & Shi, Y. (2020). Structural and Biochemical Characterization of the nsp12-nsp7-nsp8 Core Polymerase Complex from SARS-CoV-2. Cell reports. https://doi.org/10.1016/j.celrep.2020.107774

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for SARS-CoV-2 nsp8 Antibody (NBP3-07047) (0)

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Secondary Antibodies

 

Isotype Controls

Additional SARS-CoV-2 nsp8 Products

Array NBP3-07047

Bioinformatics Tool for SARS-CoV-2 nsp8 Antibody (NBP3-07047)

Discover related pathways, diseases and genes to SARS-CoV-2 nsp8 Antibody (NBP3-07047). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Omicron Variant Antibodies

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Bioinformatics

Gene Symbol ORF1ab