SARS-CoV-2 NSP8 Antibodies
SARS-CoV-2 Nonstructural Protein 8 (NSP8) is one of the sixteen nonstructural proteins of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), the causative agent of COVID-19. SARS-CoV-2 NSP8 has a theoretical molecular weight of 21.9 kDa and heterodimerizes with NSP7 which, together, forms a complex with NSP12. Additionally, NSP8 interacts with the SARS-CoV-2 open reading frame 6 (ORF6), promoting RNA polymerase activity. Browse our catalog of rabbit polyclonal SARS-CoV-2 NSP8 antibodies. Validated research applications for SARS-CoV-2 NSP8 antibodies include ELISA and Western Blot. Choose the viral-specific anti-SARS-CoV-2 NSP8 antibody that is best suited for your research needs by viewing the technical details, images, and related products. Our SARS-CoV-2 NSP8 antibodies are fully covered by Novus’ Guarantee+.
Alternate Names for SARS-CoV-2 NSP8 Antibodies
anti-Non-structural protein 8 antibody, anti-nsp8 replicase antibody
10 Results for "sars-cov-2-nsp8" in Primary Antibodies
![Western Blot: SARS-CoV-2 nsp8 Antibody [NBP3-13472] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) nsp8 antibody (NBP3-13472) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/SARS-CoV-2-nsp8-Antibody-Western-Blot-NBP3-13472-img0001.jpg "Western Blot: SARS-CoV-2 nsp8 Antibody [NBP3-13472] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) nsp8 antibody (NBP3-13472) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: SARS-CoV-2 nsp8 Antibody [NBP3-13472] - SARS-CoV-2 (COVID-19) nsp8 antibody detects SARS-CoV-2 (COVID-19) nsp8 protein at cytoplasm by immunofluorescent analysis. Sample: Mock and transfected transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SARS-CoV-2 (COVID-19) nsp8 stained by SARS-CoV-2 (COVID-19) nsp8 antibody (NBP3-13472) diluted at 1:2000.](http://images.novusbio.com/fullsize/SARS-CoV-2-nsp8-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-13472-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: SARS-CoV-2 nsp8 Antibody [NBP3-13472] - SARS-CoV-2 (COVID-19) nsp8 antibody detects SARS-CoV-2 (COVID-19) nsp8 protein at cytoplasm by immunofluorescent analysis. Sample: Mock and transfected transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SARS-CoV-2 (COVID-19) nsp8 stained by SARS-CoV-2 (COVID-19) nsp8 antibody (NBP3-13472) diluted at 1:2000.](http://images.novusbio.com/fullsize/SARS-CoV-2-nsp8-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-13472-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: SARS-CoV-2 nsp8 Antibody [NBP3-13472] - SARS-CoV-2 (COVID-19) nsp8 antibody detects SARS-CoV-2 (COVID-19) nsp8 protein at cytoplasm by immunohistochemical analysis. Sample: Mock and SARS-CoV-2 (COVID-19) nsp8 transfected 293T cell FFPE Cell Pellet Block. Green: SARS-CoV-2 (COVID-19) nsp8 stained by SARS-CoV-2 (COVID-19) nsp8 antibody (NBP3-13472) diluted at 1:1000. Blue: Fluoroshield with DAPI. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/SARS-CoV-2-nsp8-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-13472-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: SARS-CoV-2 nsp8 Antibody (HL1523) - Azide and BSA Free [NBP3-25664] - SARS-CoV-2 (COVID-19) nsp8 antibody [HL1523] detects SARS-CoV-2 (COVID-19) nsp8 protein by immunofluorescent analysis. Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SARS-CoV-2 (COVID-19) nsp8 stained by SARS-CoV-2 (COVID-19) nsp8 antibody [HL1523] (NBP3-25664) diluted at 1:500. Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-25664_rabbit-sars-cov-2-nsp8-mab-hl1523-azide-and-bsa-free-2120249425495.jpg "Immunocytochemistry/Immunofluorescence: SARS-CoV-2 nsp8 Antibody (HL1523) - Azide and BSA Free [NBP3-25664] - SARS-CoV-2 (COVID-19) nsp8 antibody [HL1523] detects SARS-CoV-2 (COVID-19) nsp8 protein by immunofluorescent analysis. Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SARS-CoV-2 (COVID-19) nsp8 stained by SARS-CoV-2 (COVID-19) nsp8 antibody [HL1523] (NBP3-25664) diluted at 1:500. Blue: Fluoroshield with DAPI .")
![Immunohistochemistry-Paraffin: SARS-CoV-2 nsp8 Antibody (HL1523) - Azide and BSA Free [NBP3-25664] - SARS-CoV-2 (COVID-19) nsp8 antibody [HL1523] detects SARS-CoV-2 (COVID-19) nsp8 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mock and SARS-CoV-2 Nsp8 transfected 293T cell pellet. Green: SARS-CoV-2 (COVID-19) nsp8 stained by SARS-CoV-2 (COVID-19) nsp8 antibody [HL1523] (NBP3-25664) diluted at 1:100. Blue: Fluoroshield with DAPI . Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25664_rabbit-sars-cov-2-nsp8-mab-hl1523-azide-and-bsa-free-2120249374970.jpg)
![Western Blot: SARS-CoV-2 nsp8 Antibody (HL1523) - Azide and BSA Free [NBP3-25664] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) nsp8 antibody [HL1523] (NBP3-25664) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25664_rabbit-sars-cov-2-nsp8-mab-hl1523-azide-and-bsa-free-212024939171.jpg)
![Western Blot: SARS-CoV-2 nsp8 Antibody [NBP3-13471] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) nsp8 antibody (NBP3-13471) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/SARS-CoV-2-nsp8-Antibody-Western-Blot-NBP3-13471-img0001.jpg "Western Blot: SARS-CoV-2 nsp8 Antibody [NBP3-13471] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) nsp8 antibody (NBP3-13471) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: SARS-CoV-2 nsp8 Antibody [NBP3-13471] - SARS-CoV-2 (COVID-19) nsp8 antibody detects SARS-CoV-2 (COVID-19) nsp8 protein at cytoplasm by immunohistochemical analysis. Sample: Mock and SARS-CoV-2 (COVID-19) nsp8 transfected 293T cell FFPE Cell Pellet Block. Green: SARS-CoV-2 (COVID-19) nsp8 stained by SARS-CoV-2 (COVID-19) nsp8 antibody (NBP3-13471) diluted at 1:1000. Blue: Fluoroshield with DAPI. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/SARS-CoV-2-nsp8-Antibody-Immunohistochemistry-Paraffin-NBP3-13471-img0002.jpg)
![Immunohistochemistry: SARS-CoV-2 nsp8 Antibody [NBP3-07046] - Immunohistochemical analysis of paraffin-embedded COVID-19 patient lung tissue using anti- SARS-CoV-2 (COVID-19) NSP8 antibody (0.5 ug/mL). Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 degrees celsius. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin. Strong signal of SARS-COV-2 NSP8 protein was observed in macrophage of COVID-19 patient lung, but not in non-COVID-19 patient lung.](http://images.novusbio.com/fullsize/SARS-CoV-2-nsp8-Antibody-Immunohistochemistry-NBP3-07046-img0001.jpg "Immunohistochemistry: SARS-CoV-2 nsp8 Antibody [NBP3-07046] - Immunohistochemical analysis of paraffin-embedded COVID-19 patient lung tissue using anti- SARS-CoV-2 (COVID-19) NSP8 antibody (0.5 ug/mL). Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 degrees celsius. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin. Strong signal of SARS-COV-2 NSP8 protein was observed in macrophage of COVID-19 patient lung, but not in non-COVID-19 patient lung.")
![ELISA: SARS-CoV-2 nsp8 Antibody [NBP3-07046] - Antbodies: SARS-CoV-2 (COVID-19) NSP8 Antibody. A direct ELISA was performed using SARS-CoV-2 NSP8 recombinant protein (10-415) as coating antigen and the anti-SARS-CoV-2 (COVID-19) NSP8 antibody as the capture antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution. Detection range is from 1 ng/mL to 3000 ng/mL](http://images.novusbio.com/fullsize/SARS-CoV-2-nsp8-Antibody-ELISA-NBP3-07046-img0002.jpg)
![Western Blot: SARS-CoV-2 nsp8 Antibody [NBP3-07047] - Western Blot Validation with SARS-CoV-2 nsp8 Protein. Loading: 30 ng per lane of SARS-CoV-2 nsp8 protein. Antibodies: SARS-CoV-2 nsp8 (NBP3-07047), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: 0.5 ug/ml and Lane 2: 1 ug/ml](http://images.novusbio.com/fullsize/SARS-CoV-2-nsp8-Antibody-Western-Blot-NBP3-07047-img0002.jpg "Western Blot: SARS-CoV-2 nsp8 Antibody [NBP3-07047] - Western Blot Validation with SARS-CoV-2 nsp8 Protein. Loading: 30 ng per lane of SARS-CoV-2 nsp8 protein. Antibodies: SARS-CoV-2 nsp8 (NBP3-07047), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: 0.5 ug/ml and Lane 2: 1 ug/ml")
![ELISA: SARS-CoV-2 nsp8 Antibody [NBP3-07047] - ELISA Validation with SARS-CoV-2 nsp8 Protein. Antibodies: SARS-CoV-2 nsp8 Antibody (NBP3-07047). A direct ELISA was performed using SARS-CoV-2 nsp8 recombinant protein as coating antigen and the anti-SARS-CoV-2 nsp8 antibody as the capture antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution. Detection range is from 0.3 ng/ml to 3000 ng/ml](http://images.novusbio.com/fullsize/SARS-CoV-2-nsp8-Antibody-ELISA-NBP3-07047-img0001.jpg)
![Western Blot: SARS-CoV-2 nsp8 Antibody [NBP3-15980] - Western blot analysis of extracts of normal 293T cells and 293T transfected with NSP8 Protein, using SARS-CoV-2 nsp8 antibody (NBP3-15980) at 1:10000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/SARS-CoV-2-nsp8-Antibody-Western-Blot-NBP3-15980-img0001.jpg "Western Blot: SARS-CoV-2 nsp8 Antibody [NBP3-15980] - Western blot analysis of extracts of normal 293T cells and 293T transfected with NSP8 Protein, using SARS-CoV-2 nsp8 antibody (NBP3-15980) at 1:10000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Western Blot: SARS-CoV-2 nsp8 Antibody [NBP3-07968] - Various lysates were subjected to SDS-PAGE followed by western blot with SARS-CoV-2 (2019-nCoV) NSP8 Protein antibody at dilution of 1:1000.](http://images.novusbio.com/fullsize/SARS-CoV-2-nsp8-Antibody-Western-Blot-NBP3-07968-img0001.jpg "Western Blot: SARS-CoV-2 nsp8 Antibody [NBP3-07968] - Various lysates were subjected to SDS-PAGE followed by western blot with SARS-CoV-2 (2019-nCoV) NSP8 Protein antibody at dilution of 1:1000.")