SARS-CoV-2 NSP10 Antibody - Azide and BSA Free

Western Blot: SARS-CoV-2 NSP10 Antibody [NBP3-16002] - Western blot analysis of extracts of normal 293T cells, 293T transfected with NSP10 Protein and 293T transfected with SOST Protein, using SARS-CoV-2 NSP10 antibody (NBP3-16002) at 1:10000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Immunoprecipitation: SARS-CoV-2 NSP10 Antibody - Azide and BSA Free [SARS-CoV-2 NSP10] - Immunoprecipitation of SARS-CoV-2 NSP10 from 300 ug extracts of 293T cells transfected with SARS-CoV-2 NSP10 was performed using 2 ug of SARS-CoV-2 NSP10 Rabbit pAb . Rabbit IgG isotype control was used to precipitate the Control IgG sample. IP samples were eluted with 1X Laemmli Buffer. The Input lane represents 10 % of the total input.Western blot analysis of immunoprecipitates was conducted using SARS-CoV-2 NSP10 Rabbit pAb at a dilution of 1:4000.

• Reactivity: V
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

SARS-CoV-2 NSP10 Antibody - Azide and BSA Free Summary

• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-139 of coronavirus NSP10 (YP_009725306.1). AGNATEVPANSTVLSFCAFAVDAAKAYKDYLASGGQPITNCVKMLCTHTGTGQAITVTPEANMDQESFGGASCCLYCRCHIDHPNPKGFCDLKGKYVQIPTTCANDPVGFTLKNTVCTVCGMWKGYGCSCDQLREPMLQ
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ORF1ab
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:2000 - 1:10000

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.05% Proclin 300
• Purity: Affinity purified

Alternate Names for SARS-CoV-2 NSP10 Antibody - Azide and BSA Free

• GFL
• Growth factor-like peptide
• Non-structural protein 10

Background

SARS-CoV-2 Nonstructural Protein 9 (NSP9) is one of the sixteen nonstructural proteins of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), the causative agent of COVID-19 (1). SARS-CoV-2 NSP9 is 113 amino acids (aa) with a theoretical molecular weight of 12.4 kDa (1-3). The amino acid sequence alignment of SARS-CoV and SARS-CoV-2's NSP9 has 97.3% sequence identity and 99.1% sequence similarity (1). Structurally NSP9 has a N-terminal beta-barrel made of seven beta-strands and a C-terminal alpha-helix (3). In SARS-CoV, NSP9 is important for viral proliferation; it is unclear if NSP9 plays the same role in SARS-CoV-2 but given the high degree of sequence similarity it is quite likely (4). SARS-CoV-2 NSP9 also plays a role in formation of nuclear transport machinery and the extracellular matrix (2). Additionally, NSP9 binds mind bomb 1 (MIB1), an E3 ubiquitin ligase that regulates antiviral innate immune signaling (2).

References

1. Yoshimoto F. K. (2020). The Proteins of Severe Acute Respiratory Syndrome Coronavirus-2 (SARS CoV-2 or n-COV19), the Cause of COVID-19. The Protein Journal. https://doi.org/10.1007/s10930-020-09901-4

2. Gordon, D. E., Jang, G. M., Bouhaddou, M., Xu, J., Obernier, K., White, K. M., O'Meara, M. J., Rezelj, V. V., Guo, J. Z., Swaney, D. L., Tummino, T. A., Huttenhain, R., Kaake, R. M., Richards, A. L., Tutuncuoglu, B., Foussard, H., Batra, J., Haas, K., Modak, M., Kim, M., ... Krogan, N. J. (2020). A SARS-CoV-2 protein interaction map reveals targets for drug repurposing. Nature. https://doi.org/10.1038/s41586-020-2286-9

3. Qiu, Y., & Xu, K. (2020). Functional studies of the coronavirus nonstructural proteins. STEMedicine. https://doi.org/10.37175/stemedicine.v1i2.39

4. Khan, M. T., Irfan, M., Ahsan, H., Ahmed, A., Kaushik, A. C., Khan, A. S., Chinnasamy, S., Ali, A., & Wei, D. Q. (2021). Structures of SARS-CoV-2 RNA-Binding Proteins and Therapeutic Targets. Intervirology. https://doi.org/10.1159/000513686

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

Bioinformatics

• Gene Symbol: ORF1ab