rRNA Antibody (Y10b) - Azide and BSA Free

Immunocytochemistry/Immunofluorescence: rRNA Antibody (Y10b) - Azide and BSA Free [NBP2-80932] - Staining of rRNA in vero cells. Image from the standard format of this antibody.

Immunohistochemistry: rRNA Antibody (Y10b) - Azide and BSA Free [NBP2-80932] - Immunohistochemical staining of mouse neuronal progenitor culture using rRNA antibody (green). Image from verified customer review. Image from the standard format of this antibody.

Flow Cytometry: rRNA Antibody (Y10b) - Azide and BSA Free [NBP2-80932] - An intracellular stain was performed on HeLa cells with rRNA Antibody [Y10b] NB100-662B (blue) and a matched isotype control (orange). Both antibodies were directly conjugated to Biotin. Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature, followed by Streptavidin - R-Phycoerythrin Protein (2012-1000, Novus Biologicals).

Immunocytochemistry/Immunofluorescence: rRNA Antibody (Y10b) - Azide and BSA Free [NBP2-80932] - HeLa cells were fixed and permeabilized for 10 minutes using -20C MeOH. The cells were incubated with anti-rRNA (Y10b) conjugated to DyLight 488 [NB100-662G] at 5ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Immunocytochemistry/Immunofluorescence: rRNA Antibody (Y10b) - Azide and BSA Free [NBP2-80932] - rRNA antibody was tested in Ntera-2 cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red). Image from the standard format of this antibody.

CyTOF-ready: rRNA Antibody (Y10b) - Azide and BSA Free [NBP2-80932] - Human bone marrow after RBC lysis, stained with anti-CD16, fixed, permeabilized with methanol, stained with anti-lactoferrin 1C6 NB120-10109 and anti-rRNA Y10b NB100-662, and gated on myeloid cells per Nat Med. 2020. 26:408-417. CyTOF image submitted by a verified customer review. Image from the standard format of this antibody.

• Reactivity: Hu, Mu, All, Ba
• Applications: Flow, ICC/IF, IHC, IP, CyTOF-ready
• Clone: Y10b
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1 mg/ml

rRNA Antibody (Y10b) - Azide and BSA Free Summary

• Immunogen: The whole 5.8S ribosomal RNA.
• Localization: Cytoplasmic. Nuclear.
• Isotype: IgG3 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow (Intracellular)
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence 1:2500
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation 10 ul/lysate
• Application Notes: This rRNA Antibody (Y10b) is useful for Immunocytochemistry/Immunofluorescence and Immunoprecipitation. In ICC/IF, staining was observed in Ribosomes in the nucleoli and cytoplasm of Ntera2 cells.

Reactivity Notes

This antibody reacts with all eukaryotes. Bacteria reactivity reported in scientific literature (PMID: 26134566).

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for rRNA Antibody (Y10b) - Azide and BSA Free

• ribosomal RNA 5.8s

Background

rRNA (ribosomal RNA) forms a major structural component of ribosomes which provides a mechanism for decoding mRNA information into amino acids/proteins through the process of translation. rRNA plays a key role in accurate positioning of mRNA as well as peptidyl tRNA, and also plays a structural role in peptidyl transferase activity by generating scaffolds where ribosomal proteins are assembled. rRNA is generated inside nucleolus, which is localized in nucleus of the cells and contains genes essential for encoding rRNA. Defective rRNA may implicate in certain pathologies, for example: decreased rRNA expression has been observed in subjects with myelodysplastic syndromes, and abnormal rRNA expression has also been linked to Alzheimer's disease. Our rRNA (Y10b) antibody recognizes both the large as well as the small subunit of rRNA from all eukaryotic cells and with is applicability in ICC/IF and IP techniques, it provides a valuable molecular tool for rRNA research.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.