![Immunocytochemistry/Immunofluorescence: RNF219 Antibody [NBP1-88401] - Staining of human cell line A-431 shows localization to nucleoplasm, nuclear bodies & cytosol.](http://images.novusbio.com/fullsize/RNF219-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-88401-img0004.jpg "Immunocytochemistry/Immunofluorescence: RNF219 Antibody [NBP1-88401] - Staining of human cell line A-431 shows localization to nucleoplasm, nuclear bodies & cytosol.")
| Reactivity | HuSpecies Glossary |
| Applications | ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Description | Novus Biologicals Rabbit RNF219 Antibody - BSA Free (NBP1-88401) is a polyclonal antibody validated for use in IHC and ICC/IF. Anti-RNF219 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee. |
| Immunogen | This antibody was developed against Recombinant Protein corresponding to amino acids: SKGSLTNDQLENGSEWKPTSFFLLSPSDQEMNEDFSLHSSSCPVTNEIKPPSCLFQTEFSQGILLSSSHRLFEDQRFGSSLFK |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | RNF219 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100. |
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| Publications |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.2) and 40% Glycerol |
| Preservative | 0.02% Sodium Azide |
| Purity | Immunogen affinity purified |
| Publication using NBP1-88401 | Applications | Species |
|---|---|---|
| Stadler C, Rexhepaj E, Singan VR et al. Immunofluorescence and fluorescent-protein tagging show high correlation for protein localization in mammalian cells. Nat Methods 2013-04-01 [PMID: 23435261] |
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | RNF219 |
![Immunohistochemistry-Paraffin: RNF219 Antibody [NBP1-88401] - Staining of human cerebral cortex.](http://images.novusbio.com/fullsize/RNF219-Antibody-Immunohistochemistry-Paraffin-NBP1-88401-img0012.jpg "Immunohistochemistry-Paraffin: RNF219 Antibody [NBP1-88401] - Staining of human cerebral cortex.")
![Immunohistochemistry-Paraffin: RNF219 Antibody [NBP1-88401] - Staining of human pancreas shows low expression as expected.](http://images.novusbio.com/fullsize/RNF219-Antibody-Immunohistochemistry-Paraffin-NBP1-88401-img0006.jpg "Immunohistochemistry-Paraffin: RNF219 Antibody [NBP1-88401] - Staining of human pancreas shows low expression as expected.")
![Immunohistochemistry-Paraffin: RNF219 Antibody [NBP1-88401] - Staining of human testis shows high expression.](http://images.novusbio.com/fullsize/RNF219-Antibody-Immunohistochemistry-Paraffin-NBP1-88401-img0007.jpg "Immunohistochemistry-Paraffin: RNF219 Antibody [NBP1-88401] - Staining of human testis shows high expression.")
![Immunohistochemistry-Paraffin: RNF219 Antibody [NBP1-88401] - Staining in human testis and pancreas tissues using anti-RNF219 antibody. Corresponding RNF219 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/RNF219-Antibody-Immunohistochemistry-Paraffin-NBP1-88401-img0008.jpg "Immunohistochemistry-Paraffin: RNF219 Antibody [NBP1-88401] - Staining in human testis and pancreas tissues using anti-RNF219 antibody. Corresponding RNF219 RNA-seq data are presented for the same tissues.")
![Immunohistochemistry-Paraffin: RNF219 Antibody [NBP1-88401] - Staining of human cerebral cortex, kidney, lymph node and testis using Anti-RNF219 antibody NBP1-88401 (A) shows similar protein distribution across tissues to independent antibody NBP2-33979 (B).](http://images.novusbio.com/fullsize/RNF219-Antibody-Immunohistochemistry-Paraffin-NBP1-88401-img0009.jpg "Immunohistochemistry-Paraffin: RNF219 Antibody [NBP1-88401] - Staining of human cerebral cortex, kidney, lymph node and testis using Anti-RNF219 antibody NBP1-88401 (A) shows similar protein distribution across tissues to independent antibody NBP2-33979 (B).")
![Immunohistochemistry-Paraffin: RNF219 Antibody [NBP1-88401] - Staining of human lymph node.](http://images.novusbio.com/fullsize/RNF219-Antibody-Immunohistochemistry-Paraffin-NBP1-88401-img0010.jpg "Immunohistochemistry-Paraffin: RNF219 Antibody [NBP1-88401] - Staining of human lymph node.")
![Immunohistochemistry-Paraffin: RNF219 Antibody [NBP1-88401] - Staining of human kidney.](http://images.novusbio.com/fullsize/RNF219-Antibody-Immunohistochemistry-Paraffin-NBP1-88401-img0011.jpg "Immunohistochemistry-Paraffin: RNF219 Antibody [NBP1-88401] - Staining of human kidney.")
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")