RNF212 Recombinant Protein Antigen

• Reactivity: Hu
• Applications: AC

RNF212 Recombinant Protein Antigen Summary

• Description: A recombinant protein antigen with a N-terminal His6-ABP tag corresponding to human RNF212.
  
  

  Source: E. coli

  Amino Acid Sequence: KLLEFIKHVCYHRHQSHRPCAPGWFCQVLQRPGAVSGEKTQQTRPAPPATCLLCLSCLSGFRHGPWRSQALPSDLVAPLFVSYTVEVSITNAGWS

  Fusion Tag: N-terminal His6ABP (ABP = Albumin Binding Protein derived from Streptococcal Protein G)

  This product is intended to be used as a blocking antigen for antibody competition assays. Any other use of this antigen is done at the risk of the user. The use of this product for commercial production is strictly prohibited. Please contact technical support if you have any questions.

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein Antigen
• Gene: RNF212
• Purity: >80% by SDS-PAGE and Coomassie blue staining

Applications/Dilutions

• Dilutions:
  • Antibody Competition 10 - 100 molar excess
• Application Notes: This recombinant antigen is only intended to be used as a blocking agent to confirm antibody specificity with the corresponding antibody, catalog number NBP2-49259.

  It is purified by IMAC chromatography, and the expected concentration is greater than 0.5 mg/ml.

  For current lot information, including availability, please contact our technical support team click nb-technical@bio-techne.com

• Theoretical MW: 28 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS and 1M Urea, pH 7.4.
• Preservative: No Preservative
• Purity: >80% by SDS-PAGE and Coomassie blue staining

Alternate Names for RNF212 Recombinant Protein Antigen

• FLJ38841
• FLJ42587
• hypothetical protein LOC285498
• MGC120227
• MGC120228
• ring finger protein 212
• ZHP3

Background

RNF212, also known as Probable E3 SUMO-protein ligase RNF212, has 6 isoforms, a 297 amino acid isoform that is 33 kDa, a 123 amino acid isoform that is 14 kDa, a 192 amino acid isoform that is 22 kDa, a 280 amino acid isoform that is 31 kDa, a 232 amino acid isoform that is 26 kDa, and a 273 amino acid isoform that is 30 kDa. This protein may function as a ubiquitin ligase and may be involved in meiotic recombination, regulates crossing-over during meiosis: required to couple chromosome synapsis to the formation of crossover-specific recombination complexes, localizes to recombination sites and stabilizes meiosis-specific recombination factors, may act as a SUMO E3 ligase that mediates sumoylation of target proteins MSH4 and/or MSH5, leading to enhance their binding to recombination sites, and acts as a limiting factor for crossover designation and/or reinforcement.RNF212 protein is being studied for its involvement in recombination rate qtl 1 disorder. Little is currently known about this protein interactions with other proteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: RNF212