RG9MTD3 Antibody

Images

 
Western Blot: RG9MTD3 Antibody [NBP1-88770] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Negative control (vector only transfected HEK293T lysate). Lane 3: Over-expression lysate (Co-expressed ...read more
Immunocytochemistry/ Immunofluorescence: RG9MTD3 Antibody [NBP1-88770] - Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.
Immunohistochemistry-Paraffin: RG9MTD3 Antibody [NBP1-88770] - Staining of human esophagus shows moderate cytoplasmic positivity in squamous epithelial cells.
Immunocytochemistry/ Immunofluorescence: RG9MTD3 Antibody [NBP1-88770] - Staining of human cell line A-431 shows positivity in nucleus but not nucleoli, plasma membrane and cell junctions.
Immunohistochemistry-Paraffin: RG9MTD3 Antibody [NBP1-88770] - Staining of human prostate shows distinct positivity in basal cells.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

RG9MTD3 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids:KVYILGGLVDESIQKKVTFQKAREYSVKTARLPIQEYMVRNQNGKNYHSEILAINQVFDILSTYLETHNWPEALKKGVSSGKGYILRNSV
Specificity
Specificity of antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TRMT10B
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:500
  • Immunocytochemistry/Immunofluorescence 1 - 4 ug/ml
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50-1:200
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
RG9MTD3 Protein (NBP1-88770PEP)

Reactivity Notes

Expected species cross reactivity based on sequence homology: Mouse (86%), Rat (86%)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for RG9MTD3 Antibody

  • bA3J10.9
  • EC 2.1.1
  • EC 2.1.1.-
  • FLJ31455
  • RNA (guanine-9-) methyltransferase domain containing 3
  • RNA (guanine-9-)-methyltransferase domain-containing protein 3
  • RP11-3J10.9

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for RG9MTD3 Antibody (NBP1-88770) (0)

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Secondary Antibodies

 

Isotype Controls

Additional RG9MTD3 Products

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Bioinformatics

Gene Symbol TRMT10B