Recombinant Mouse VSTM2A His-tag Protein, CF

2 μg/lane of Recombinant Mouse VSTM2A was resolved with SDS-PAGE underreducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Bluestaining, showing bands at 35-44 kDa.

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse VSTM2A His-tag Protein, CF Summary

• Details of Functionality: Measured by its ability to inhibit anti-CD3 antibody induced IFN-gamma secretion by human peripheral blood mononuclear cells (PBMC). The ED₅₀ for his effect is 2-12 μg/mL.
• Source: Mouse myeloma cell line, NS0-derived mouse VSTM2A protein
  Ser25-Ala252, with a C-terminal 6-His tag
• Accession #: NP_001277468.1
• N-terminal Sequence: Ser25
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 26 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 35-44 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage:
  • 12 months from date of receipt, ≤ -20 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 100 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse VSTM2A His-tag Protein, CF

• MGC33530
• V-set and transmembrane domain containing 2
• V-set and transmembrane domain containing 2A
• V-set and transmembrane domain-containing protein 2A
• VSTM2
• VSTM2A

Background

V-set and transmembrane domain-containing protein 2A (VSTM2A) is a secreted glycoprotein that is expressed by committed preadipocytes. N-linked glycosylation is crucial for its secretion, but not for preadipocyte cell differentiation activity. It is expressed during adipocyte development and its over-expression promotes adipogenesis (1). VSTM2A is highly expressed in the brain and Vstm2a was identified as an enigmatic gene that is highly produced in mouse brain (1, 2). A positive association has been observed between Vstm2a and Pparg2. PPAR gamma 2 indirectly promotes Vstm2a expression in preadipocytes by amplifying adipogenic commitment, while VSTM2A promotes Pparg2 expression by activating BMP signaling (1, 3). VSTM2A is synthesized as a precursor protein that contains a 24 amino acid (aa) signal sequence followed by the VSTM2A domain. The mouse VSTM2A shares 78% and 99% aa sequence identity with human and rat VSTM2A, respectively. Adipogenic commitment was uninhibited by over-expression of VSTM2A lacking a signal peptide for secretion, suggesting that secreted VSTM2A is not involved in adipogenic commitment in vitro. While the exact role secreted VSTM2A plays is unknown, it is suggested it may modulate angiogenesis or neurogenesis due to its expression in the brain and near blood vessels, and the need for adipose tissue to develop alongside blood vessels and neural tissue (1, 4). Our data shows that mouse VSTM2A inhibits anti-CD3 induced IFN-gamma secretion in Human T cells.

1. Secco, B. et al. (2017) Cell Rep. 18:93.
2. Pandey, A.K. et al. (2014) PloS One. 9:e88889.
3. Berry, D.C. et al. (2013) Development. 140:3939.
4. Nishimura, S. et al. (2007) Diabetes. 56:1517.

Bioinformatics

• Uniprot:
  • NP_001277468.1()