Pichia Pastoris Antibody

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Product Details

Summary
Reactivity YeSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-Fr
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
4.0 mg/ml

Order Details

Pichia Pastoris Antibody Summary

Immunogen
P. pastoris whole cells
Specificity
Reacts with all Pichia pastoris antigens and displays cross reactivity with C. albicans and to a lesser extent S. cerevisiae. This is useful for detection/removal of contaminants from recombinant preparations.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Purity
IgG purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2)
Preservative
0.1% Sodium Azide
Concentration
4.0 mg/ml
Purity
IgG purified

Background

Pichia pastoris, a methylotrophic yeast, has been used as a heterologous protein expression system. It combines many of the benefits of E. coli expression with the advantages of expression in an eukaryotic system such as protein processing, folding, and posttranslational modifications. Pichia pastoris is capable of metabolizing methanol as the sole carbon source. The first step in the metabolism of methanol is the oxidation of methanol to formaldehyde by the enzyme, alcohol oxidase. Expression of this enzyme, coded for by the AOX1 gene, is tightly regulated and induced by methanol to very high levels: typically >30% of the total soluble protein in the cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

Additional Pichia Pastoris Products

Pichia Pastoris NB100-65067

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