PI 4 Kinase type 2 beta Antibody - BSA Free

Western Blot: PI 4 Kinase type 2 beta Antibody [NBP1-87908] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Negative control (vector only transfected HEK293T lysate). Lane 3: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).

Immunocytochemistry/Immunofluorescence: PI 4 Kinase type 2 beta Antibody [NBP1-87908] - Immunofluorescent staining of human cell line A-431 shows localization to cytosol.

Immunohistochemistry-Paraffin: PI 4 Kinase type 2 beta Antibody [NBP1-87908] - Staining of human prostate shows moderate cytoplasmic positivity in smooth muscle cells.

Immunohistochemistry-Paraffin: PI 4 Kinase type 2 beta Antibody [NBP1-87908] - Staining of human colon shows moderate cytoplasmic and membranous positivity in glandular cells.

Immunohistochemistry-Paraffin: PI 4 Kinase type 2 beta Antibody [NBP1-87908] - Staining of human duodenum shows moderate to strong membranous positivity in glandular cells.

Immunohistochemistry-Paraffin: PI 4 Kinase type 2 beta Antibody [NBP1-87908] - Staining of human lymph node shows moderate to strong membranous positivity in non-germinal and germinal center cells.

Immunohistochemistry-Paraffin: PI 4 Kinase type 2 beta Antibody [NBP1-87908] - Staining of human pancreas shows very weak positivity in exocrine glandular cells as expected.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

PI 4 Kinase type 2 beta Antibody - BSA Free Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: LILPYISDMNFVQDLCEDLYELFKTDKGFDKATFESQMSVMRGQILNLTQALRDGKSPFQLVQIPCVIVERSQGGSQGRIVHLSNSFTQTVNCRKPF
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PI4K2B
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  PI 4 Kinase type 2 beta Protein (NBP1-87908PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (87%), Rat (86%)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for PI 4 Kinase type 2 beta Antibody - BSA Free

• EC 2.7.1.67
• FLJ11105
• phosphatidylinositol 4-kinase type 2 beta
• phosphatidylinositol 4-kinase type 2-beta
• Phosphatidylinositol 4-kinase type II-beta
• phosphatidylinositol 4-kinase type-II beta
• PI 4 Kinase type 2 beta
• PI4K2B
• PI4KIIB
• PI4KII-BETA
• PIK42B

Background

Phosphatidylinositol 4-kinases (PI4Ks) phosphorylate phosphatidylinositol to generate phosphatidylinositol 4-phosphate (PIP), an immediate precursor of several important signaling and scaffolding molecules. PIP itself may also have direct functional and structural roles. PI4K2B is a primarily cytosolic PI4K that is recruited to membranes, where it stimulates phosphatidylinositol 4,5-bisphosphate synthesis (Wei et al., 2002 (PubMed 12324459)).(supplied by OMIM)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PI4K2B