PI 4 Kinase type 2 beta Antibody

Images

 
Western Blot: PI 4 Kinase type 2 beta Antibody [NBP1-87908] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Negative control (vector only transfected HEK293T lysate). Lane 3: Over-expression lysate ...read more
Immunocytochemistry/ Immunofluorescence: PI 4 Kinase type 2 beta Antibody [NBP1-87908] - Immunofluorescent staining of human cell line A-431 shows localization to cytosol.
Immunohistochemistry-Paraffin: PI 4 Kinase type 2 beta Antibody [NBP1-87908] - Staining of human prostate shows moderate cytoplasmic positivity in smooth muscle cells.
Immunohistochemistry-Paraffin: PI 4 Kinase type 2 beta Antibody [NBP1-87908] - Staining of human colon shows moderate cytoplasmic and membranous positivity in glandular cells.
Immunohistochemistry-Paraffin: PI 4 Kinase type 2 beta Antibody [NBP1-87908] - Staining of human duodenum shows moderate to strong membranous positivity in glandular cells.
Immunohistochemistry-Paraffin: PI 4 Kinase type 2 beta Antibody [NBP1-87908] - Staining of human lymph node shows moderate to strong membranous positivity in non-germinal and germinal center cells.
Immunohistochemistry-Paraffin: PI 4 Kinase type 2 beta Antibody [NBP1-87908] - Staining of human pancreas shows very weak positivity in exocrine glandular cells as expected.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

PI 4 Kinase type 2 beta Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: LILPYISDMNFVQDLCEDLYELFKTDKGFDKATFESQMSVMRGQILNLTQALRDGKSPFQLVQIPCVIVERSQGGSQGRIVHLSNSFTQTVNCRKPF
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PI4K2B
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
PI 4 Kinase type 2 beta Protein (NBP1-87908PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (87%), Rat (86%)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for PI 4 Kinase type 2 beta Antibody

  • EC 2.7.1.67
  • FLJ11105
  • phosphatidylinositol 4-kinase type 2 beta
  • phosphatidylinositol 4-kinase type 2-beta
  • Phosphatidylinositol 4-kinase type II-beta
  • phosphatidylinositol 4-kinase type-II beta
  • PI 4 Kinase type 2 beta
  • PI4K2B
  • PI4KIIB
  • PI4KII-BETA
  • PIK42B

Background

Phosphatidylinositol 4-kinases (PI4Ks) phosphorylate phosphatidylinositol to generate phosphatidylinositol 4-phosphate (PIP), an immediate precursor of several important signaling and scaffolding molecules. PIP itself may also have direct functional and structural roles. PI4K2B is a primarily cytosolic PI4K that is recruited to membranes, where it stimulates phosphatidylinositol 4,5-bisphosphate synthesis (Wei et al., 2002 (PubMed 12324459)).(supplied by OMIM)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol PI4K2B