phycoerythrin Antibody (PE-DLF) [Biotin]

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Flow Cytometry: phycoerythrin Antibody (PE-DLF) [Biotin] [NBP2-00145] - Staining of C57BL/6 splenocytes with Anti Human/Mouse CD45R (B220) PE and 0.06 ug of Mouse IgG1 kappa Isotype Control Biotin (left) or 0.06 ug of ...read more

Product Details

Summary
Reactivity NASpecies Glossary
Applications Flow
Clone
PE-DLF
Clonality
Monoclonal
Host
Mouse
Conjugate
Biotin
Concentration
0.5 mg/ml

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Catalog# & Conjugate Size Price

phycoerythrin Antibody (PE-DLF) [Biotin] Summary

Immunogen
The immunogen for this antibody is Phycoerythrin.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:10-1:1000
Application Notes
This PE-DLF antibody has been tested by flow cytometric analysis. This can be used at less than or equal to 0.125 ug per test. A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 uL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Readout System

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Aqueous buffer, may contain carrier protein/stabilizer (proprietary)
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein A or G purified

Alternate Names for phycoerythrin Antibody (PE-DLF) [Biotin]

  • PE
  • R-PE

Background

The PE-DLF monoclonal antibody reacts with phycoerythrin (PE), PE-Cy5 and PE-Cy7, with no crossreactivity to fluorescein (FITC) or allophycocyanin (APC). When using PE-DLF as a second-step antibody, in combination with an appropriate third step, to bind a PEconjugated primary antibody, there is no decrease in the fluorescent intensity of the directly conjugated PE antibody. Therefore no interference or quenching of the PE fluorochrome is observed. The PE-DLF can be used for separation of cells labeled with PE-conjugated antibodies or staining. Please note that the optimal concentration for this antibody will vary depending on the density of the primary PE-conjugated antibody and the application tested.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

Isotype Controls

Additional phycoerythrin Products

phycoerythrin NBP2-00145

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