phycoerythrin Antibody (PE-DLF) [Biotin]


Flow Cytometry: phycoerythrin Antibody (PE-DLF) [Biotin] [NBP2-00145] - Staining of C57BL/6 splenocytes with Anti Human/Mouse CD45R (B220) PE and 0.06 ug of Mouse IgG1 kappa Isotype Control Biotin (left) or 0.06 ug of more

Product Details

Reactivity NASpecies Glossary
Applications Flow, ICC/IF, IHC
0.5 mg/ml

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phycoerythrin Antibody (PE-DLF) [Biotin] Summary

The immunogen for this antibody is Phycoerythrin.
IgG1 Kappa
Protein A or G purified
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  • Flow Cytometry 1:10-1:1000
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
Application Notes
This PE-DLF antibody has been tested by flow cytometric analysis. This can be used at less than or equal to 0.125 ug per test. A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 uL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Readout System

Packaging, Storage & Formulations

Store at 4C in the dark.
PBS (pH 7.2) with 0.1% gelatin
0.09% Sodium Azide
0.5 mg/ml
Protein A or G purified

Alternate Names for phycoerythrin Antibody (PE-DLF) [Biotin]

  • PE
  • R-PE


The PE-DLF monoclonal antibody reacts with phycoerythrin (PE), PE-Cy5 and PE-Cy7, with no crossreactivity to fluorescein (FITC) or allophycocyanin (APC). When using PE-DLF as a second-step antibody, in combination with an appropriate third step, to bind a PEconjugated primary antibody, there is no decrease in the fluorescent intensity of the directly conjugated PE antibody. Therefore no interference or quenching of the PE fluorochrome is observed. The PE-DLF can be used for separation of cells labeled with PE-conjugated antibodies or staining. Please note that the optimal concentration for this antibody will vary depending on the density of the primary PE-conjugated antibody and the application tested.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

ICC/IF Video Protocol

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Secondary Antibodies


Isotype Controls

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