phycoerythrin Antibody (PE-DLF)


Flow Cytometry: phycoerythrin Antibody (PE-DLF) [NBP2-00146] - Analysis using the Biotin conjugate of NBP2-00146. Staining of C57BL/6 splenocytes with Anti Human/Mouse CD45R (B220) PE and 0.06 ug of Mouse IgG1 kappa more
Flow Cytometry: phycoerythrin Antibody (PE-DLF) [NBP2-00146] - Staining of normal human peripheral blood cells with Rat Anti-Human/Mouse CD44 PE, followed by 0.125 ug of Mouse IgG1 K Isotype Control Purified (left) or more

Product Details

Reactivity NASpecies Glossary
Applications Flow
0.5 mg/ml

Order Details

Available Conjugates
Catalog# & Conjugate Size Price

phycoerythrin Antibody (PE-DLF) Summary

The immunogen for this antibody is Phycoerythrin.
IgG1 Kappa
Protein A or G purified
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Packaging, Storage & Formulations

Store at 4C. Do not freeze.
Aqueous buffer, may contain carrier protein/stabilizer (proprietary).
0.09% Sodium Azide
0.5 mg/ml
Protein A or G purified


Application Notes
This PE-DLF antibody has been tested by flow cytometric analysis. This can be used at less than or equal to 0.125 ug per test. A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 uL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Alternate Names for phycoerythrin Antibody (PE-DLF)

  • PE
  • R-PE


The PE-DLF monoclonal antibody reacts with phycoerythrin (PE), PE-Cy5 and PE-Cy7, with no crossreactivity to fluorescein (FITC) or allophycocyanin (APC). When using PE-DLF as a second-step antibody, in combination with an appropriate third step, to bind a PEconjugated primary antibody, there is no decrease in the fluorescent intensity of the directly conjugated PE antibody. Therefore no interference or quenching of the PE fluorochrome is observed. The PE-DLF can be used for separation of cells labeled with PE-conjugated antibodies or staining. Please note that the optimal concentration for this antibody will vary depending on the density of the primary PE-conjugated antibody and the application tested.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies


Isotype Controls

Other Available Formats

Biotin Labeled NBP2-00145

Additional phycoerythrin Antibody (PE-DLF) Products

phycoerythrin NBP2-00146

Blogs on phycoerythrin

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