![Western Blot: Phosphothreonine Antibody [NB110-96896] - Western blot analysis of Mouse brain cell lysates showing detection of Phosphothreonine protein using Rabbit Anti-Phosphothreonine Polyclonal Antibody (NB110-96896). Primary Antibody: Rabbit Anti-Phosphothreonine Polyclonal Antibody (NB110-96896) at 1:1000. Left: Treated with Vanadium, Right: Non-treated..](http://images.novusbio.com/fullsize/Phosphothreonine-Antibody-Western-Blot-NB110-96896-img0007.jpg "Western Blot: Phosphothreonine Antibody [NB110-96896] - Western blot analysis of Mouse brain cell lysates showing detection of Phosphothreonine protein using Rabbit Anti-Phosphothreonine Polyclonal Antibody (NB110-96896). Primary Antibody: Rabbit Anti-Phosphothreonine Polyclonal Antibody (NB110-96896) at 1:1000. Left: Treated with Vanadium, Right: Non-treated..")
| Reactivity | All-NASpecies Glossary |
| Applications | WB, ELISA, ICC/IF, IP |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 0.25 mg/ml |
| Description | Novus Biologicals Rabbit Phosphothreonine Antibody - BSA Free (NB110-96896) is a polyclonal antibody validated for use in WB, ELISA, ICC/IF and IP. Anti-Phosphothreonine Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee. |
| Immunogen | Phosphothreonine conjugated to KLH |
| Specificity | Recognizes proteins phosphorylated on threonine residues. Does not cross-react with phosphotyrosine. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Purity | Peptide affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | PBS, 50% Glycerol |
| Preservative | 0.01% Sodium Azide |
| Concentration | 0.25 mg/ml |
| Purity | Peptide affinity purified |
| Publication using NB110-96896 | Applications | Species |
|---|---|---|
| Alavi MV Tau phosphorylation and OPA1 proteolysis are unrelated events: Implications for Alzheimer's Disease Biochim Biophys Acta Mol Cell Res 2021-08-17 [PMID: 34400176] (WB, Mouse) | WB | Mouse |
| Images | Ratings | Applications | Species | Date | Details | ||||||||||
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reviewed by:
Marcel Alavi |
WB | Mouse | 02/22/2021 |
Summary
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reviewed by:
Verified Customer |
WB | Rat | 09/19/2019 |
Summary
Comments
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Secondary Antibodies |
Isotype Controls |
Research Areas for Phosphothreonine Antibody (NB110-96896)Find related products by research area.
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Phosphotyrosine: A Global Player in Human Health and Disease Protein tyrosine phosphorylation is a fundamental mechanism for controlling many aspects of cellular processes, as well as aspects of human health and diseases. Compared with phosphoserine and phosphothreonine, phosphotyrosine signaling is more tightl... Read full blog post. |
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Phosphotyrosine: Affecting Locally Acting Globally Over the past two decades, it has become clear that tyrosine phosphorylation plays a pivotal role in a variety of important signaling pathways in multicellular organisms. In the typical vertebrate cell, phosphotyrosine represents only a tiny fraction ... Read full blog post. |
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Phosphoserine: A Basic Cellular Signal with Wide Ranging Effects Phosphoserine is an ester of serine and phosphoric acid which results from posttranslational modifications. Phosphorylation is a key post-translational modification necessary for normal cellular signaling. Phosphorylated proteins mediate cell division... Read full blog post. |
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Phosphotyrosine is Critical Signal Transduction and Regulation Phosphotyrosine is the phosphorylated version of the amino acid tyrosine, which results from the activation of intracellular protein kinases (e.g. via growth factors) during normal growth and development, well as in transformation and oncogenesis. Pho... Read full blog post. |
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| Marcel Alavi 02/22/2021 |
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| Application: | WB | |
| Species: | Mouse |
| Verified Customer 09/19/2019 |
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| Application: | WB | |
| Species: | Rat |
![Immunocytochemistry/Immunofluorescence: Phosphothreonine Antibody [NB110-96896] - Human HeLa cells. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-Phosphothreonine Polyclonal Antibody at 1:60 for 12 hours at 4C. Secondary Antibody: R-PE Goat Anti-Rabbit (yellow) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Nucleus. Magnification: 20x.](http://images.novusbio.com/fullsize/Phosphothreonine-Antibody-Immunocytochemistry-Immunofluorescence-NB110-96896-img0005.jpg "Immunocytochemistry/Immunofluorescence: Phosphothreonine Antibody [NB110-96896] - Human HeLa cells. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-Phosphothreonine Polyclonal Antibody at 1:60 for 12 hours at 4C. Secondary Antibody: R-PE Goat Anti-Rabbit (yellow) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Nucleus. Magnification: 20x.")
![Immunocytochemistry/Immunofluorescence: Phosphothreonine Antibody [NB110-96896] - Human HeLa cells. Fixation: 2% Formaldehyde for 20 min at RT. Rabbit Anti-Phosphothreonine Polyclonal Antibody at 1:60 for 12 hours at 4C. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Nucleus. Magnification: 100x.](http://images.novusbio.com/fullsize/Phosphothreonine-Antibody-Immunocytochemistry-Immunofluorescence-NB110-96896-img0004.jpg "Immunocytochemistry/Immunofluorescence: Phosphothreonine Antibody [NB110-96896] - Human HeLa cells. Fixation: 2% Formaldehyde for 20 min at RT. Rabbit Anti-Phosphothreonine Polyclonal Antibody at 1:60 for 12 hours at 4C. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Nucleus. Magnification: 100x.")
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")
