Peripheral Node Addressin Antibody (MECA-79) - Low Endotoxin and Azide Free

Western Blot: Peripheral Node Addressin Antibody (MECA-79) - Low Endotoxin and Azide Free [NBP2-78790] - Total protein from human Tonsil, Lymph node, Spleen and mouse Spleen was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-PNAd in blocking buffer and detected with an anti-rat HRP secondary antibody using West Pico PLUS chemiluminescence detection reagent. Image using the standard format of this product.

Immunohistochemistry-Paraffin: Peripheral Node Addressin Antibody (MECA-79) - Low Endotoxin and Azide Free [NBP2-78790] - Analysis of FFPE mouse adipose tissue section (with lymph node areas) using Peripheral Node Addressin antibody (clone MECA-79) at 1:100. The staining was developed with HRP-DAB detection method and the counterstaining was performed using hematoxylin. This Peripheral Node Addressin antibody generated a strong and specific staining of MECA-79 antigen in the the cytoplasm and the membranes of high endothelial venules (HEVs) aka peripheral lymph node addressin (PNAd) in lymph node areas of tested adipose tissue section. Image using the standard format of this product.

Immunohistochemistry: Peripheral Node Addressin Antibody (MECA-79) - Low Endotoxin and Azide Free [NBP2-78790] - Analysis of FFPE human tonsil tissue section using Peripheral Node Addressin antibody (clone MECA-79R) at 1:100. The staining was developed with HRP-DAB detection method and the counterstaining was performed using hematoxylin. Image using the standard format of this product.

• Reactivity: Hu, Mu
• Applications: WB, Func, IHC, IP
• Clone: MECA-79
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Format: Low Endotoxin and Azide Free
• Concentration: 1.0 mg/ml

Peripheral Node Addressin Antibody (MECA-79) - Low Endotoxin and Azide Free Summary

• Immunogen: Mouse lymph node stromal cells
• Isotype: IgM Kappa
• Clonality: Monoclonal
• Host: Rat
• Purity: IgM purified
• Endotoxin Note: Endotoxin level is <0.02 EU/ug

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100-1:500
  • Immunohistochemistry-Frozen 1:100-1:500
  • Immunohistochemistry-Paraffin 1:100-1:500
  • Immunoprecipitation 1:10-1:500
  • In vivo assay reported in scientific literature (PMID 30277476)
  • Western Blot 1:100-1:2000
• Application Notes: Additional reported application of in vitro and in vivo blocking of cell adhesion.

Packaging, Storage & Formulations

• Storage: Store at -20 degrees C. Avoid freeze/thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Concentration: 1.0 mg/ml
• Purity: IgM purified

Alternate Names for Peripheral Node Addressin Antibody (MECA-79) - Low Endotoxin and Azide Free

• MECA-79
• Peripheral Node Addressin
• PNAd

Background

MECA-79 recognizes a carbohydrate epitope shared with a group of sulfation decorated sialomucins, including sulfated ligands for CD62L (CD34, GlyCAM-1, Sgp200, and a subset of MAdCAM-1). This set antigens has been referred to as peripheral node addressin (PNAd) with the molecular mass 50-250 kD. It has been identified that GlcNAc-6-SO4 sulfation contributes to MECA-79 binding and the core 1 beta1,3-N-acetylglucosaminyltransferase is required for the generation of the MECA-79 epitope. MECA-79 is expressed on high endothelial venules (HEV) of lymphoid tissues, chronic inflamed tissues and rheumatoid synovia. The interaction of PNAd with CD62L receptor is involved in tethering and rolling of lymphocytes along HEV in lymphoid tissues. MECA-79 antibody reacts with mouse, human and many other species PNAd and blocks L-selectin-dependent lymphocyte adhesion in vitro and in vivo.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.