Western Blot: Peripheral Node Addressin Antibody (MECA-79) [NB100-77673] - Total protein from Human tonsil (lane 1) and human lymph node (lane 2) was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and ...read more
Alternate Names for Peripheral Node Addressin Antibody (MECA-79)
Peripheral Node Addressin
MECA-79 recognizes a carbohydrate epitope shared with a group of sulfation decorated sialomucins, including sulfated ligands for CD62L (CD34, GlyCAM-1, Sgp200, and a subset of MAdCAM-1). This set antigens has been referred to as peripheral node addressin (PNAd) with the molecular mass 50-250 kD. It has been identified that GlcNAc-6-SO4 sulfation contributes to MECA-79 binding and the core 1 beta1,3-N-acetylglucosaminyltransferase is required for the generation of the MECA-79 epitope. MECA-79 is expressed on high endothelial venules (HEV) of lymphoid tissues, chronic inflamed tissues and rheumatoid synovia. The interaction of PNAd with CD62L receptor is involved in tethering and rolling of lymphocytes along HEV in lymphoid tissues. MECA-79 antibody reacts with mouse, human and many other species PNAd and blocks L-selectin-dependent lymphocyte adhesion in vitro and in vivo.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Publications for Peripheral Node Addressin Antibody (NB100-77673)(11)
We have publications tested in 2 confirmed species: Human, Mouse.
We have publications tested in 2 applications: IHC, IHC-P.
FAQs for Peripheral Node Addressin Antibody (NB100-77673). (Showing 1 - 3 of 3 FAQs).
What is the recommended concentration for the use of this antibody on human paraffin sections, as well as a specific protocol for this antibody?
We do not have a specific protocol for this product, and we recommend a starting dilution of 1:100-1:200 for IHC-P. We performed heat induced antigen retrieval with Citrate buffer pH6.0.
We are very interested in your MECA-79 antibody. The catalog# is NB100-77673. We want to inject the antibody to mouse as blocking antibody. Our question is whether the antibody solution contains toxic sodium azide? Thanks.
NB100-77673 does contain 0.09% sodium azide. We do offer antibody cleanup kits which you might find useful as they remove unwanted substances such as sodium azide.
I am searching for an antibody against MECA 79 that reacts with human, mouse primary cells for WB or IP-WB. I did use one other vendor's, but it hard for me to use that for WB. Since my project will use MECA 79 antibody a lot, so I found today that you have this antibody. I would like to try it. My question is: Do you have the image picture for WB?
Unfortunately, we do not have a western blot image of this product. We do have a publication that used this antibody in western blot among other immunoassays. It is currently not available on our website, but you may view it using this link: PMC3003387. We also do not have a smaller size of this antibody available. I am sorry for any inconvenience. We will fully guarantee all listed applications and species.