Peripheral Node Addressin Antibody (MECA-79)

Images

 
Immunohistochemistry-Paraffin: Peripheral Node Addressin Antibody (MECA-79) [NB100-77673] - IHC analysis of a formalin-fixed paraffin-embedded (FFPE) mouse adipose tissue section (with lymph node areas) using Peripheral ...read more
Western Blot: Peripheral Node Addressin Antibody (MECA-79) [NB100-77673] - Total protein from Human tonsil (lane 1) and human lymph node (lane 2) was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IHC, IHC-Fr, IHC-P, IP
Clone
MECA-79
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Peripheral Node Addressin Antibody (MECA-79) Summary

Immunogen
Mouse lymph node stromal cells
Isotype
IgM Kappa
Clonality
Monoclonal
Host
Rat
Purity
IgM purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • Immunohistochemistry 1:100-1:500
  • Immunohistochemistry-Frozen 1:100-1:500
  • Immunohistochemistry-Paraffin 1:100-1:500
  • Immunoprecipitation 1:10-1:500
Application Notes
Additional reported application of in vitro and in vivo blocking of cell adhesion.
Publications
Read Publications using
NB100-77673 in the following applications:

Reactivity Notes

Human and mouse.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Tris-NaCl, pH 8.5
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
IgM purified

Alternate Names for Peripheral Node Addressin Antibody (MECA-79)

  • MECA-79
  • Peripheral Node Addressin
  • PNAd

Background

MECA-79 recognizes a carbohydrate epitope shared with a group of sulfation decorated sialomucins, including sulfated ligands for CD62L (CD34, GlyCAM-1, Sgp200, and a subset of MAdCAM-1). This set antigens has been referred to as peripheral node addressin (PNAd) with the molecular mass 50-250 kD. It has been identified that GlcNAc-6-SO4 sulfation contributes to MECA-79 binding and the core 1 beta1,3-N-acetylglucosaminyltransferase is required for the generation of the MECA-79 epitope. MECA-79 is expressed on high endothelial venules (HEV) of lymphoid tissues, chronic inflamed tissues and rheumatoid synovia. The interaction of PNAd with CD62L receptor is involved in tethering and rolling of lymphocytes along HEV in lymphoid tissues. MECA-79 antibody reacts with mouse, human and many other species PNAd and blocks L-selectin-dependent lymphocyte adhesion in vitro and in vivo.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Peripheral Node Addressin Antibody (NB100-77673)(11)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 2 applications: IHC, IHC-P.


Filter By Application
IHC
(2)
IHC-P
(1)
All Applications
Filter By Species
Human
(1)
Mouse
(2)
All Species
Showing Publications 1 - 10 of 11. Show All 11 Publications.
Publications using NB100-77673 Applications Species
Hautz T, Zelger BG, Nasr IW et al. Lymphoid Neogenesis in Skin of Human Hand, Nonhuman Primate and Rat Vascularized Composite Allografts. Transpl. Int. 2014 May 23 [PMID: 24853399] (IHC-P, Human) IHC-P Human
Hirakawa J, Tsuboi K, Sato K et al. Novel anti-carbohydrate antibodies reveal the cooperative function of sulfated N- and O-glycans in lymphocyte homing. J Biol Chem. 2010 Dec. [PMID: 20929857]
Burke SD, Dong H, Hazan AD et al. Aberrant endometrial features of pregnancy in diabetic NOD mice. Diabetes. 2007 Dec. [PMID: 17827401]
Drayton DL, Ying X, Lee J et al. Ectopic LT alpha beta directs lymphoid organ neogenesis with concomitant expression of peripheral node addressin and a HEV-restricted sulfotransferase. J Exp Med. 2003 May 5 [PMID: 12732657] (IHC, Mouse) IHC Mouse
Samulowitz U, Kuhn A, Brachtendorf G et al. Human endomucin: distribution pattern, expression on high endothelial venules, and decoration with the MECA-79 epitope. Am J Pathol. 2002 May [PMID: 12000719]
Csencsits KL, Jutila MA, Pascual DW. Nasal-associated lymphoid tissue: phenotypic and functional evidence for the primary role of peripheral node addressin in naive lymphocyte adhesion to high endothelial venules in a mucosal site. J Immunol. 1999 Aug 1 [PMID: 10415038] (IHC, Mouse) IHC Mouse
Michie SA, Streeter PR, Bolt PA et al. The human peripheral lymph node vascular addressin. An inducible endothelial antigen involved in lymphocyte homing. Am J Pathol. 1993 Dec. [PMID: 8256856]
Hemmerich S, Butcher EC, Rosen SD et al. Sulfation-dependent recognition of high endothelial venules (HEV)-ligands by L-selectin and MECA 79, and adhesion-blocking monoclonal antibody. J Exp Med. 1994 Dec. [PMID: 7525849]
Berg EL, McEvoy LM, Berlin C et al. L-selectin-mediated lymphocyte rolling on MAdCAM-1. Nature. 1993 Dec. [PMID: 7505053]
Streeter PR, Rouse BT, Butcher EC et al. Immunohistologic and functional characterization of a vascular addressin involved in lymphocyte homing into peripheral lymph nodes. J Cell Biol. 1988 Nov. [PMID: 2460470]
Show All 11 Publications.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Peripheral Node Addressin Antibody (NB100-77673). (Showing 1 - 3 of 3 FAQs).

  1. What is the recommended concentration for the use of this antibody on human paraffin sections, as well as a specific protocol for this antibody?
    • We do not have a specific protocol for this product, and we recommend a starting dilution of 1:100-1:200 for IHC-P. We performed heat induced antigen retrieval with Citrate buffer pH6.0.
  2. We are very interested in your MECA-79 antibody. The catalog# is NB100-77673. We want to inject the antibody to mouse as blocking antibody. Our question is whether the antibody solution contains toxic sodium azide? Thanks.
    • NB100-77673 does contain 0.09% sodium azide. We do offer antibody cleanup kits which you might find useful as they remove unwanted substances such as sodium azide.
  3. I am searching for an antibody against MECA 79 that reacts with human, mouse primary cells for WB or IP-WB. I did use one other vendor's, but it hard for me to use that for WB. Since my project will use MECA 79 antibody a lot, so I found today that you have this antibody. I would like to try it. My question is: Do you have the image picture for WB?
    • Unfortunately, we do not have a western blot image of this product. We do have a publication that used this antibody in western blot among other immunoassays. It is currently not available on our website, but you may view it using this link: PMC3003387. We also do not have a smaller size of this antibody available. I am sorry for any inconvenience. We will fully guarantee all listed applications and species.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB100-77673AF405
Alexa Fluor 488 Labeled NB100-77673AF488
Alexa Fluor 647 Labeled NB100-77673AF647
Alexa Fluor 700 Labeled NB100-77673AF700
Biotin Labeled NB100-77673B
DyLight 350 Labeled NB100-77673UV
DyLight 405 Labeled NB100-77673V
DyLight 488 Labeled NB100-77673G
DyLight 550 Labeled NB100-77673R
DyLight 650 Labeled NB100-77673C
DyLight 680 Labeled NB100-77673FR
DyLight 755 Labeled NB100-77673IR
HRP Labeled NB100-77673H

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