p35 Antibody

Images

 
Western Blot: p35 Antibody [NBP2-21650] - Detection of 0.1 ug of recombinant p35 protein. Lane 1: Molecular weight markers. Lane 2: MBP-p35 fusion protein. Lane 3: MBP alone. Protein was run on a 4-20% gel, then ...read more

Product Details

Summary
Reactivity BaSpecies Glossary
Applications WB, ELISA
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

p35 Antibody Summary

Immunogen
MBP-fusion protein corresponding to Borrelia burgdorferi p35 protein. Protein accession number NP_045522.1.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
BB_H32
Purity
Protein A purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • ELISA 1:5000
Application Notes
Tested for use in Western blotting and ELISA. Expect a band approximately 27.1 kDa in size corresponding to Borrelia burgdorferi OspA protein by Western blotting in the appropriate cell lysate or extract.

Reactivity Notes

Reactive in Borrelia burgdorferi. A BLAST analysis was used to suggest cross-reactivity with p35 from B. burgdorferi, garinii, and afzelii sources based on 100% identity with the immunizing sequence.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Concentration
LYOPH
Purity
Protein A purified
Reconstitution Instructions
Reconstitute with 0.1 ml deionized water.

Alternate Names for p35 Antibody

  • BB_H32

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

Additional p35 Products

p35 NBP2-21650

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Bioinformatics

Gene Symbol BB_H32