Otolin-1 Antibody (839418) [Alexa Fluor® 532]

• Applications: WB, ICC/IF
• Clone: 839418
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 532

Otolin-1 Antibody (839418) [Alexa Fluor® 532] Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant human Otolin-1
  Lys24-Pro477
  Accession # A6NHN0
• Specificity: Detects human Otolin-1 in ELISAs and Western blots.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Background

Otolin (OTOL1), also known as C1qTNF15, is an approximately 65 kDa protein found in the otoconial membrane lining the cochlea and vestibular labyrinth of the inner ear. It is secreted by supporting cells of the sensory epithelium. The otoconial membrane contains particles known as otoconia which are composed of glycoproteins and proteoglycans coated with calcium carbonate crystals. Otolin is one of the protein components of otoconia particles, and it is important for otoconia formation as well as for auditory and vestibular function. It associates into multimers and disulfide-linked oligomers and also associates with other otoconial proteins including Cerebellin-1 and Otoconin-90. Otolin contains three collagen-like regions followed by a C1q-like domain at the C-terminus. It is extensively glycosylated and has multiple hydroxylated proline residues in the collagenous regions.  Human Otolin shares 72% aa sequence identity with mouse and rat Otolin.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.