OSGIN2 Antibody (OTI7D12) - Azide and BSA Free

Western Blot: OSGIN2 Antibody (OTI7D12) - Azide and BSA Free [NBP2-73164] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY OSGIN2, (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with antiOSGIN2.(1:2000)

Immunohistochemistry: OSGIN2 Antibody (OTI7D12) - Azide and BSA Free [NBP2-73164] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using antiOSGIN2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 degrees C for 3 min,)(1:150)

Immunohistochemistry: OSGIN2 Antibody (OTI7D12) - Azide and BSA Free [NBP2-73164] - Staining of paraffin-embedded Carcinoma of Human liver tissue using anti-OSGIN2 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 degrees C for 3 min,)(1:150)

Immunohistochemistry: OSGIN2 Antibody (OTI7D12) - Azide and BSA Free [NBP2-73164] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-OSGIN2 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 degrees C for 3 min,)(1:150)

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clone: OTI7D12
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

OSGIN2 Antibody (OTI7D12) - Azide and BSA Free Summary

• Immunogen: Human recombinant protein fragment corresponding to amino acids 1-250 of human OSGIN2(NP_004328) produced in E.coli.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: OSGIN2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Western Blot 1:2000
• Theoretical MW: 56.5 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for OSGIN2 Antibody (OTI7D12) - Azide and BSA Free

• hT41C8orf1chromosome 8 open reading frame 1
• oxidative stress induced growth inhibitor family member 2
• oxidative stress-induced growth inhibitor 2

Background

OSGIN2 may be involved in meiosis or the maturation of germ cells

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: OSGIN2