OLLAS Epitope Tag Antibody (L2) - BSA Free

Western Blot: OLLAS Epitope Tag Antibody (L2) [NBP1-06713] - C. elegans whole nematode. OLLAS::PRMT-1 Western Blot using NBP1-06713SS (1:1000). Western blot image submitted by a verified customer review.

Immunocytochemistry/Immunofluorescence: OLLAS Epitope Tag Antibody (L2) [NBP1-06713] - Oncohistone incorporation patterns induce changes in nuclear PRC2 distribution and sterility phenotypes. Live cell imaging of GFP-tagged MES-2/EZH2 (the catalytic subunit of C. elegans PRC2), and immunofluorescence of H3K27me3 and H3.3/oncohistone (tagged with OLLAS Epitope Tag) in pachytene nuclei of H3-like K27M oncohistone (H3-like mut) worms. Scale bars represent 5 um. Chromosome X was identified by depletion of H3.3 and H3K4me3 staining shown in Supplementary Fig. 3, and is marked with an asterisk. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-019-10404-9) licensed under a CC-BY license Image using the Biotin format of this antibody.

OLLAS-Epitope-Tag-Antibody-L2-BSA-Free-Immunohistochemistry-NBP1-06713-img0010.jpg

Western Blot: OLLAS Epitope Tag Antibody (L2) [NBP1-06713] - Comparison of binding sensitivity of Novus Biologicals' monoclonal antibodies to OLLAS (NBP1-06713), DYKDDDDK (NBP1-06712) and the FLAG-M2 monoclonal antibody from Sigma-Aldrich. FLAG (TM) and ANTI-FLAG (TM) are registered trademarks of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co.

Western Blot: OLLAS Epitope Tag Antibody (L2) [NBP1-06713] - OLLAS tagged SFO-p41 protein.

Immunohistochemistry Whole-Mount: OLLAS Epitope Tag Antibody (L2) [NBP1-06713] - Drosophila adult midgut expressing an OLLAS-tagged protein stained with OLLAS Epitope Tag Antibody (magenta). Nuclei are shown in green. IHC image submitted by a verified customer review.

Immunohistochemistry-Frozen: OLLAS Epitope Tag Antibody (L2) [NBP1-06713] - Vector Map of pCMV-SD OLLAS

OLLAS-Epitope-Tag-Antibody-L2-Immunohistochemistry-Paraffin-NBP1-06713-img0008.jpg

OLLAS-Epitope-Tag-Antibody-L2-Immunohistochemistry-Paraffin-NBP1-06713-img0007.jpg

Western Blot: OLLAS Epitope Tag Antibody (L2) - BSA Free [NBP1-06713] - Utilization of pMVP for the creation of unique PDX1 vectors. pENTR plasmids containing cDNA for human PDX1 with or without a stop codon (i.e. open) were recombined with pMVP components to generate an assortment of PDX1-expressing (A) adenovirus & (C) expression plasmid vectors. (B) Immunoblot blot analysis of INS1 832/13 cell lysates harvested 48h after treatment with crude adenovirus lysates. For the epitope tagged conditions, note the appearance of the endogenous (lower) & overexpressed (upper) PDX1 bands (top blot). For eGFP blot, the use of P2A adds 23 amino acids to the N-terminal protein (i.e. eGFP). Immunoblot analysis of HEK293 cell lysates 24 h after transfection of expression vectors encoding PDX1 with C-terminal (D) epitope tags, (E) eGFP reporter or fusion, (G) & mCherry reporter or fusion. (F, H) Fluorescence microscopy of the eGFP & mCherry containing conditions analyzed in panels E & G. Visible bands resulting from ‘Uncleaved’ P2A products (red circle •) & degradation products (*) are labeled. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30590691), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

MES proteins are expressed in nos-1nos-2 embryonic PGCs.Transcriptome comparison between PGCs isolated from wild-type and mes-2(RNAi) L1 larvae. (A) Volcano plot showing log2 fold change of gene expression between mes-2(RNAi) and wild-type L1 PGCs. The numbers of genes whose expression were up or downregulated in mes-2(RNAi) compared to wild-type L1 PGCs are indicated. Dashed lines mark the significance cutoff of q = 0.05 above which genes were counted as misexpressed. (B) Bar graph showing chromosomal distribution of mes-2(RNAi) upregulated genes. Asterisks indicate significantly more genes than expected (hypergeometric test, p-value<0.001 [**]). (C) Top: Photomicrograph of live embryo expressing GFP tagged MES-2 in wild- type and nos-1(gv5)nos-2(ax3103) embryos. Middle: Photomicrograph of fixed wild-type and nos-1(gv5)nos-2(ax3103) embryos expressing OLLAS tagged MES-3. Bottom: Photomicrograph of fixed wild-type and nos-1(gv5)nos-2(RNAi) embryos stained with alpha -MES-4 antibody and K76 alpha -PGL-1 antibody. Images of 2-fold+ stage embryos were taken. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/29111977), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Comparison of localization of the CD19::Nrx, nSyb::CD19 and CD19::sdc ligands into presynaptic sites in the ORNs targeting DA1.Different intracellular and transmembrane domains (from Nrx, nSyb, and sdc) were fused to CD19 and expressed into ORNs targeting the DA1 glomerulus using the CD19::Nrx (top panels), nSyb::CD19 (middle panels) and CD19::Sdc (bottom panels), with the R17H02 driver (in green). The brain samples were co-immunostained with antibodies against the OLLAS tag (present in the ligand) and against the pre-synaptic protein, BRP (in magenta). The ligand proteins are co-localized with or adjacent to BRP (arrows), demonstrating that all ligands are enriched at the presynaptic terminals of the ORNs, but CD19::Nrx was also expressed at strong levels in the axon shaft outside of the glomerulus (arrowheads in top panels). Scale bar = 20 μm. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/29231171), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

• Reactivity: ET
• Applications: WB, ICC/IF, IHC, IP, WB, ChIP, IHC
• Clone: L2
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

OLLAS Epitope Tag Antibody (L2) - BSA Free Summary

• Immunogen: OLLAS (SGFANELGPRLMGK)-tagged extracellular domain of mouse Langerin
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Rat
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation Sequencing reported in scientific literature (PMID 31175278)
  • Immunoblotting reported in scientific literature (PMID 27600537)
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry Whole-Mount
  • Immunohistochemistry 1:10 - 1:100
  • Immunohistochemistry-Frozen 1:10 - 1:100
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation 1:10 - 1:500
  • Western Blot 1:500 - 1:1000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for OLLAS Epitope Tag Antibody (L2) - BSA Free

• E.coli OmpF Linker and mouse Langerin fusion Sequence
• OLLA-2

Background

OLLAS (Escherichia coli OmpF Linker and mouse Langerin fusion Sequence) epitope tag is a relatively newly discovered tag which is a 14 amino acid sequence residing in the junction between E. coli OmpF protein (OFL/OmpF linker) and mLangerin extracellular domain. OLLAS Epitope Tag antibody (L2), also called MAb L2 or OLLA-2, is a novel rat IgG monoclonal antibody raised against newly identified OLLAS epitope (SGFANELGPRLMGK), with advantages in immunodetection applications. Additionally, this antibody is a rat IgG and can be used for immunostaining of mouse and human samples without direct labelling of fluorochrome or enzyme. Because anti-tag antibodies are mostly generated from mouse and rabbit, the OLLAS Epitope Tag antibody (L2) from rat IgG provides an extra advantage in immunodetection.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for OLLAS Epitope Tag Antibody (NBP1-06713)(55)

Publications using NBP1-06713

• Piscitello A, Nilsson A, Hawgood M et al. Temporal control of human DNA replication licensing by CDK4/6-RB signalling and chemical genetics. Nature Communications 2025-09-16 [PMID: 40940326]
• Fisher K, Lai S, Doe C Imp and Chinmo are required for embryonic motor neuron axon and dendrite targeting. Biology Open 2025-07-29 [PMID: 40720095]
• Zhifei Zhang, Haiyan Yang, Lei Fang, Guangrong Zhao, Jun Xiang, Jialin C. Zheng, Zhao Qin DOS-3 mediates cell-non-autonomous DAF-16/FOXO activity in antagonizing age-related loss of C. elegans germline stem/progenitor cells Nature Communications 2024-06-08 [PMID: 38851828]
• Niklas Simon, Abu Safyan, George Pyrowolakis, Shinya Matsuda, Erika A Bach, Utpal Banerjee Dally is not essential for Dpp spreading or internalization but for Dpp stability by antagonizing Tkv-mediated Dpp internalization eLife 2024-01-24 [PMID: 38265865]
• Piero Sanfilippo, Alexander J Kim, Anuradha Bhukel, Juyoun Yoo, Pegah S Mirshahidi, Vijaya Pandey, Harry Bevir, Ashley Yuen, Parmis S Mirshahidi, Peiyi Guo, Hong-Sheng Li, James A Wohlschlegel, Yoshinori Aso, S Lawrence Zipursky Mapping of multiple neurotransmitter receptor subtypes and distinct protein complexes to the connectome. Neuron 2024-01-13 [PMID: 38262414]
• Lea WA, Winklhofer T, Zelenchuk L et al. Polycystin-1 Interacting Protein-1 (CU062) Interacts with the Ectodomain of Polycystin-1 (PC1) Cells 2023-08-29 [PMID: 37681898] (Immunoprecipitation, Western Blot)
• Schwartz I, Vunjak M, Budroni V et al. SPOP targets the immune transcription factor IRF1 for proteasomal degradation eLife 2023-08-25 [PMID: 37622993] (WB)
• Sa Geng, Takashi Hamaji, Patrick J Ferris, Minglu Gao, Yoshiki Nishimura, James Umen A conserved RWP-RK transcription factor VSR1 controls gametic differentiation in volvocine algae. Proceedings of the National Academy of Sciences of the United States of America 2023-07-14 [PMID: 37436957]
• Thomas L, Taleb Ismail B, Askjaer P, Seydoux G Nucleoporin foci are stress-sensitive condensates dispensable for C. elegans nuclear pore assembly The EMBO journal 2023-07-03 [PMID: 37254647] (IHC-WhMt)
  
  Details:
  Dilution 1:250
• Bauer M, Aguilar G, Wharton KA et al. Heterodimerization-dependent secretion of bone morphogenetic proteins in Drosophila Developmental cell 2023-04-24 [PMID: 37054707] (IHC)
• Gleason RJ, Guo Y, Semancik CS et al. Developmentally programmed histone H3 expression regulates cellular plasticity at the parental-to-early embryo transition Science advances 2023-04-07 [PMID: 37027463] (ICC/IF)
• Podraza-Farhanieh A, Raj D, Kao G, Naredi P A proinsulin-dependent interaction between ENPL-1 and ASNA-1 in neurons is required to maintain insulin secretion in C. elegans Development (Cambridge, England) 2023-03-15 [PMID: 36939052] (WB)
• Fernando LM, Quesada-Candela C, Murray M et al. Proteasomal subunit depletions differentially affect germline integrity in C. elegans Frontiers in Cell and Developmental Biology 2022-08-17 [PMID: 36060813] (Western Blot, Immunocytochemistry/ Immunofluorescence, Human, Mouse, Hamster - Cricetulus (Chinese Hamster))
• Li S, Han S, Zhang Q et al. FUNDC2 promotes liver tumorigenesis by inhibiting MFN1-mediated mitochondrial fusion Nature communications 2022-06-17 [PMID: 35710796] (ICC/IF)
• Rudra Prasanna Banerjee, Martin Srayko Sperm-specific glycogen synthase kinase 3 is required for sperm motility and the post-fertilization signal for female meiosis II in Caenorhabditis elegans. Development (Cambridge, England) 2022-06-02 [PMID: 35635101]
• Jeon H, Lee H, Kwon DH et al. Topographic connectivity and cellular profiling reveal detailed input pathways and functionally distinct cell types in the subthalamic nucleus Cell reports 2022-03-01 [PMID: 35235786] (IF/IHC)
• Matsuda S, Schaefer JV, Mii Y et al. Asymmetric requirement of Dpp/BMP morphogen dispersal in the Drosophila wing disc Nature Communications 2021-11-08 [PMID: 34750371] (Western Blot, Immunocytochemistry/ Immunofluorescence, Human, Mouse, Hamster - Cricetulus (Chinese Hamster))
• Wenda JM, Pros�e RF, Gabus C, Steiner FA. Mitotic chromosome condensation requires phosphorylation of the centromeric protein KNL-2 in C. elegans Journal of Cell Science 2021-12-01 [PMID: 34734636]
• Prosee Rf, Wenda Jm, Ozdemir I Et Al. Transgenerational inheritance of centromere identity requires the CENP-A N-terminal tail in the C. elegans maternal germ line PLoS biology 2021-07-01 [PMID: 34228701] (WB)
• Schmidt H, Putnam A, Rasoloson D, Seydoux G Protein-based condensation mechanisms drive the assembly of RNA-rich P granules eLife 2021-06-09 [PMID: 34106046] (IF/IHC)
• Robinson-Thiewes S, Dufour B, Martel PO, Lechasseur X et Al. Non-autonomous regulation of germline stem cell proliferation by somatic MPK-1/MAPK activity in C. elegans Cell Rep 2021-05-26 [PMID: 34038716]
• Choi CP, Tay RJ, Starostik MR et al. SNPC-1.3 is a sex-specific transcription factor that drives male piRNA expression in C. elegans eLife 2021-02-15 [PMID: 33587037]
• Berkyurek AC, Furlan G, Lampersberger L et al. The RNA polymerase II subunit RPB-9 recruits the integrator complex to terminate Caenorhabditis elegans piRNA transcription The EMBO journal 2021-03-01 [PMID: 33533030] (WB)
• Hefel A, Cronin N, Harrel K et al. RPA complexes in Caenorhabditis elegans meiosis; unique roles in replication, meiotic recombination and apoptosis Nucleic Acids Res 2021-01-21 [PMID: 33476370]
• Podraza-Farhanieh A, Natarajan B, Raj D et al. ENPL-1, the Caenorhabditis elegans homolog of GRP94, promotes insulin secretion via regulation of proinsulin processing and maturation Development 2020-10-27 [PMID: 33037039]
• Sorensen EB, Seidel HS, Crittenden SL et al. A toolkit of tagged glp-1 alleles reveals strong glp-1 expression in the germline, embryo, and spermatheca MicroPubl Biol 2020-06-22 [PMID: 32626848] (Western Blot, Immunocytochemistry/ Immunofluorescence, Human, Mouse, Hamster - Cricetulus (Chinese Hamster))
• Sorensen EB, Seidel HS, Crittenden SL et al. A toolkit of tagged glp-1 alleles reveals strong glp-1 expression in the germline, embryo, and spermatheca MicroPubl Biol Jun 22 2020 [PMID: 32626848] (E. coli)
• Ouyang, J P T, Folkmann, A Et al. P Granules Protect RNA Interference Genes from Silencing by piRNAs. Dev Cell 2019-09-23 [PMID: 31402283] (WB)
• Delaney K, Strobino M, Wenda JM et al H3.3K27M-induced chromatin changes drive ectopic replication through misregulation of the JNK pathway in C. elegans Nat Commun 2019-06-09 [PMID: 31175278] (WB, Chip Cytometry, C. elegans)
• K Hacker, S Benke, B Agerer, S Scinicarie, V Budroni, GA Versteeg A repetitive acidic region contributes to the extremely rapid degradation of the cell-context essential protein TRIM52 Sci Rep, 2019-05-27;9(1):7901. 2019-05-27 [PMID: 31133683] (Western Blot, Immunocytochemistry/ Immunofluorescence, Human, Mouse, Hamster - Cricetulus (Chinese Hamster))
• Simon N, Safyan A, Pyrowolakis G, Matsuda S Dally is not essential for Dpp spreading or internalization but for Dpp stability by antagonizing Tkv-mediated Dpp internalization bioRxiv 2023-03-22 (MS)
• Takemura M, Noborn F, Nilsson J et al. Chondroitin sulfate proteoglycan Windpipe modulates Hedgehog signaling in Drosophila Molecular Biology of the Cell 2020-04-01 [PMID: 32049582] (Western Blot, Immunocytochemistry/ Immunofluorescence, Human, Mouse, Hamster - Cricetulus (Chinese Hamster))
• Chondroitin sulfate proteoglycan Windpipe modulates Hedgehog signaling in Drosophila. Takemura M, Noborn F, Nilsson J et al. Mol Biol Cell [PMID: 32049582] (IHC, Drosophila)
• Lee CS, Putnam A, Lu T et al. Recruitment of mRNAs to P granules by condensation with intrinsically-disordered proteins Elife 2020-01-24 [PMID: 31975687]
• Fujie T, Takenaka F, Yoshida E et al. A repetitive acidic region contributes to the extremely rapid degradation of the cell-context essential protein TRIM52 Sci Rep May 27 2019 [PMID: 31133683] (WB, Human)
• Sales, EC;Heckman, EL;Warren, TL;Doe, CQ; Regulation of subcellular dendritic synapse specificity by axon guidance cues Elife 2019-04-23 [PMID: 31012844] (IF/IHC)
• Sen SQ, Chanchani S, Southall TD, Doe CQ. Neuroblast-specific open chromatin allows the temporal transcription factor, Hunchback, to bind neuroblast-specific loci Elife 2019-01-29 [PMID: 30694180] (IF/IHC)
• Haldeman, J M, Conway, A E Et al. Creation of versatile cloning platforms for transgene expression and dCas9-based epigenome editing. Nucleic Acids Res 2019-02-28 [PMID: 30590691] (ELISA)
• Chen YH, Narimatsu Y, Clausen TM et al. The GAGOme: a cell-based library of displayed glycosaminoglycans Nat. Methods 2018-08-13 [PMID: 30104636] (ICC/IF)
• Huang TH, Niesman P, Arasu D et al. Tracing neuronal circuits in transgenic animals by transneuronal control of transcription (TRACT) Elife 2017-12-12 [PMID: 29231171] (ICC/IF)
• Lee CS, Lu T, Seydoux G. Nanos promotes epigenetic reprograming of the germline by down-regulation of the THAP transcription factor LIN-15B. Elife 2017 Nov 07 [PMID: 29111977] (IHC-Fr, C. elegans)
• Lee CS, Lu T, Seydoux G. Nanos promotes epigenetic reprograming of the germline by down-regulation of the THAP transcription factor LIN-15B eLife 2017-11-07 [PMID: 29111977] (Western Blot, Immunocytochemistry/ Immunofluorescence, Human, Mouse, Hamster - Cricetulus (Chinese Hamster))
• Akay A, Di Domenico T, Suen KM, Nabih A et Al. The Helicase Aquarius/EMB-4 Is Required to Overcome Intronic Barriers to Allow Nuclear RNAi Pathways to Heritably Silence Transcription Dev Cell 2017-08-09 [PMID: 28787591]
• Paix A, Folkmann A, Seydoux G. Precision genome editing using CRISPR-Cas9 and linear repair templates in C. elegans Methods 2017-05-01 [PMID: 28392263] (Western Blot, Immunocytochemistry/ Immunofluorescence, Human, Mouse, Hamster - Cricetulus (Chinese Hamster))
• Paix A, Folkmann A, Seydoux G. Precision genome editing using CRISPR-Cas9 and linear repair templates in C. elegans. Methods. Apr 7 2017 [PMID: 28392263] (ICC/IF, WB, C. elegans)
• Smith J, Calidas D, Schmidt H et al. Spatial patterning of P granules by RNA-induced phase separation of the intrinsically-disordered protein MEG-3 Elife 2016-12-03 [PMID: 27914198] (WB)
• Geng S, Hamaji T, Ferris P et al. A conserved RWP-RK transcription factor VSR1 controls gametic differentiation in volvocine algae bioRxiv 2023-03-27
• Couso I, Evans B, Li J et al. Synergism between inositol polyphosphates and TOR kinase signaling in nutrient sensing, growth control and lipid metabolism in Chlamydomonas. Plant Cell 2016-09-06 [PMID: 27600537] (IB)
• Monaghan RM, Barnes RG, Fisher K et al. A nuclear role for the respiratory enzyme CLK-1 in regulating mitochondrial stress responses and longevity Nature Cell Biology 2015-06-01 [PMID: 25961505] (Western Blot, Immunocytochemistry/ Immunofluorescence, Human, Mouse, Hamster - Cricetulus (Chinese Hamster))
• Monaghan RM, Barnes RG, Fisher K et al. A nuclear role for the respiratory enzyme CLK-1 in regulating mitochondrial stress responses and longevity. Nat. Cell Biol. 2015 Jun 01 [PMID: 25961505] (WB, Human)
• Jerlstrom-Hultqvist J, Einarsson E, Xu F et al. Hydrogenosomes in the diplomonad Spironucleus salmonicida. Nat Commun. 2013-01-01 [PMID: 24042146] (WB)
• Beckett K, Monier S, Palmer L et al. Drosophila S2 Cells Secrete Wingless on Exosome-Like Vesicles but the Wingless Gradient Forms Independently of Exosomes Traffic 2012 Oct 5 [PMID: 23035643] (Drosophila)
• Jerlstrom-Hultqvist J, Einarsson E, Svard SG. Stable transfection of the diplomonad parasite Spironucleus salmonicida Eukaryot Cell 2012 Sep 14 [PMID: 22983987] (ICC/IF, Parasite)
• Idoyaga, J et al. Cutting edge: langerin/CD207 receptor on dendritic cells mediates efficient antigen presentation on MHC I and II products in vivo. J Immunol;180(6):3647-50. 2008 Mar 15. [PMID: 18322168] (WB, Mouse)
• Park, SH et al. Generation and application of new rat monoclonal antibodies against synthetic FLAG and OLLAS tags for improved immunodetection. J Immunol Methods;331(1-2):27-38. 2008 Feb 29. [PMID: 18054954] (WB, ICC/IF, Human, Chinese Hamster, Mouse)

Reviews for OLLAS Epitope Tag Antibody (NBP1-06713) (2)

Average Rating: 4.5

(Based on 2 reviews)

We have 2 reviews tested in 2 species: C. elegans, Drosophila.

reviewed by: Verified Customer WB C. elegans 06/26/2020

Summary

• Application: Western Blot
• Sample Tested: whole C. elegans nematode
• Species: C. elegans
• Lot: F-8

reviewed by: Masahiko Takemura Immunohistochemistry-Whole mount Drosophila 12/12/2016

Summary

• Application: Other
• Sample Tested: Drosophila adult midgut
• Species: Drosophila
• Lot: F-1

FAQs for OLLAS Epitope Tag Antibody (NBP1-06713). (Showing 1 - 2 of 2 FAQs).

1. I am interested in tagging proteins from E. coli and am wondering if ompF cross-reactivity is a problem?
   • The OLLAS tag is not predicted to cross-react with any portion of ompF.
2. Can you confirm if you measure the concentration of NBP1-06713 (anti-OLLAS epitope tag)?
   • Unfortunately, we do not measure the concentration of our OLLAS tag antibody, as the product is provided unpurified.