Nuclear Pore Complex Protein Nsp1p Antibody (32D6)

Western Blot: Nuclear Pore Complex Protein Nsp1p Antibody [NBP1-05227] - Western Blot analysis of whole yeast protein extracts detects Nuclear Pore Complex Protein Nsp1p. Molecular weight detected is 86.5 kDa.

• Reactivity: Ye
• Applications: WB, ICC/IF, IHC, IP
• Clone: 32D6
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

Nuclear Pore Complex Protein Nsp1p Antibody (32D6) Summary

• Immunogen: Yeast nuclear preparations
• Marker: Nuclear Envelope Marker, Nuclear Pore Marker
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity: Tissue culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:1000-1:10000
  • Immunohistochemistry 1:1000-1:10000
  • Immunoprecipitation
  • Western Blot 1:10000-1:50000
• Application Notes: This Nuclear Pore Complex Protein Nsp1p (32D6) antibody is useful for Western blot, Immunocytochemistry/Immunofluorescence, and Immunohistochemistry
• Theoretical MW: 86.5 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

S. cerevisiae

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Supplied as concentrated hybridoma cell culture media.
• Preservative: 0.035% Sodium Azide
• Purity: Tissue culture supernatant

Alternate Names for Nuclear Pore Complex Protein Nsp1p Antibody (32D6)

• 107 kDa nucleoporin
• NPC proteins
• Nuclear pore complex protein Nup107
• Nucleoporin 107kDa
• Nucleoporin Nup107
• NUP 107
• NUP 84
• NUP107
• NUP153
• NUP155
• NUP84
• NUP98

Background

Nsp1p represents one of the most abundant nucleoporins (Nups) and is the only Nups member known to form two distinct NPC subcomplexes which are (i) Nic96p: this complex is composed of Nsp1p, Nup57p, Nup49p, and Nic96p, and is localized to both sides of the central gated channel and to the nuclear basket (ii) Nup82p: this complex is consists of Nsp1p, Nup82p, and Nup159p and is found exclusively on the cytoplasmic phase of nuclear pore complex. Moreover, Nup82p complex has also been documented to associate with Nup116p-Gle2p subcomplex. Both of these Nsp1p complexes (Nic96p and Nup82p) are implicated in delivering critical roles in nucleo-cytoplasmic transport. Genetic mutation or induced depletion of Nsp1p as well as of Nic96p, Nup57p, or Nup49p results in defective protein import. Nup82 or Nup159 mutants depict an expediated onset of nuclear mRNA retention with no impact on nuclear protein import. Moreover, Nsp1p, Nup49p and Nic96p appear to implicate in export of ribosomal large subunits, whereas Nup49 mutants are impaired in mRNA export.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.