NOP9 Overexpression Lysate (Denatured) Summary
| Description |
Plasmid: pCMV-C14orf21 full-length |
| Gene |
NOP9 |
Packaging, Storage & Formulations
| Storage |
Store at -80C. Avoid freeze-thaw cycles. |
| Buffer |
Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, and 0.01% Bromophenol blue). |
Lysate Details for NOP9
| Type |
Overexpression |
| Protein State |
Denatured |
Notes
Quality control test: Transient overexpression cell lysate was tested with Anti-C14orf21 antibody by Western Blots.
This product is produced by and distributed for Abnova, a company based in Taiwan.
Alternate Names for NOP9 Overexpression Lysate (Denatured)
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are
guaranteed for 6 months from date of receipt.
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Product General Protocols
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Blogs on NOP9
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![Immunocytochemistry/Immunofluorescence: NM23-H2/NME2 Antibody (1D3) [H00004831-M06] - Analysis of monoclonal antibody to NME2 on HeLa cell. Antibody concentration 10 ug/ml](http://images.novusbio.com/fullsize2/NM23-H2-NME2-Antibody-(1D3)-Immunocytochemistry-Immunofluorescence-H00004831-M06-img0009.jpg)
![Immunohistochemistry-Paraffin: NM23-H2/NME2 Antibody (1D3) [H00004831-M06] - Analysis of monoclonal antibody to NME2 on formalin-fixed paraffin-embedded human testis. Antibody concentration 1.5 ug/ml](http://images.novusbio.com/fullsize2/NM23-H2-NME2-Antibody-(1D3)-Immunohistochemistry-Paraffin-H00004831-M06-img0011.jpg)
![Western Blot: MyoD Antibody (5.8A) [NB100-56511] - Analysis for MyoD expression in various small round cell tumor lines using 1 ug/ml anti-MyoD mAb. The antibody only reacts with a band of approx. 45 kD in the rhabdomyosarcoma cell line (Rh30, lane 1) but was negative against the primitive neuroectodermal (PFSK-1A, lane 2), lymphoma (EB2, lane 3), neuroblastoma (SK-N-SH, lane 4), and Ewing's sarcoma (SJSA-1, lane 5) cell lines.](http://images.novusbio.com/fullsize2/MyoD-Antibody-(5.8A)-Western-Blot-NB100-56511-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: MyoD Antibody (5.8A) [NB100-56511] - RD cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-MyoD1 (5.8A) NB100-56511 at a 1:200 dilution overnight at 4C and detected with and anti-mouse Dylight 488 (Green) at a 1:500 dilution. Actin was counterstained with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize2/MyoD-Antibody-(5.8A)-Immunocytochemistry-Immunofluorescence-NB100-56511-img0004.jpg)
![Immunohistochemistry: MyoD Antibody (5.8A) [NB100-56511] - IHC (F) using the MyoD1, Clone 5.8A antibody in human tissues. A. Rhabdomyosaroma (nuclei are stained), B. Lymphoma (staining is absent)](http://images.novusbio.com/fullsize2/MyoD-Antibody-(5.8A)-Immunohistochemistry-NB100-56511-img0005.jpg)
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![Immunocytochemistry/Immunofluorescence: MUC-1 Antibody [NBP1-60046] - MUC1 antibody (NBP1-60046) was tested in HeLa cells at a 1:50 dilution against Dylight 488 (Green). Cells were counterstained for alpha-tubulin against Dylight 550 (Red) and mounted in DAPI fluoromount (Blue).](http://images.novusbio.com/fullsize2/MUC-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-60046-img0010.jpg)
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![Western Blot: MyoD Antibody (5.8A) [NB100-56511] - Analysis for MyoD expression in various small round cell tumor lines using 1 ug/ml anti-MyoD mAb. The antibody only reacts with a band of approx. 45 kD in the rhabdomyosarcoma cell line (Rh30, lane 1) but was negative against the primitive neuroectodermal (PFSK-1A, lane 2), lymphoma (EB2, lane 3), neuroblastoma (SK-N-SH, lane 4), and Ewing's sarcoma (SJSA-1, lane 5) cell lines.](http://images.novusbio.com/images2/MyoD-Antibody-(5.8A)-Western-Blot-NB100-56511-img0006.jpg "Western Blot: MyoD Antibody (5.8A) [NB100-56511] - Analysis for MyoD expression in various small round cell tumor lines using 1 ug/ml anti-MyoD mAb. The antibody only reacts with a band of approx. 45 kD in the rhabdomyosarcoma cell line (Rh30, lane 1) but was negative against the primitive neuroectodermal (PFSK-1A, lane 2), lymphoma (EB2, lane 3), neuroblastoma (SK-N-SH, lane 4), and Ewing's sarcoma (SJSA-1, lane 5) cell lines.")
![Western Blot: NOP9 Antibody [NBP2-15634] - NOP9 Sample (30 ug of whole cell lysate) A: A549 7. 5% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/images2/NOP9-Antibody-Western-Blot-NBP2-15634-img0004.jpg "Western Blot: NOP9 Antibody [NBP2-15634] - NOP9 Sample (30 ug of whole cell lysate) A: A549 7. 5% SDS PAGE gel, diluted at 1:1000.")
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