NMNAT3 Antibody

Product Discontinued

NMNAT3 Antibody Summary

• Immunogen: Peptide with sequence C-GSTWKGKSTQSTE corresponding to C-Terminus according to NP_835471.1.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: NMNAT3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:32000
• Application Notes: WB: Preliminary experiments gave an approx. 48 kDa band in human brain (cerebral cortex, frontal cortex, hippocampus) lysates after 0.3 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 24.1 kDa band according to NP_835471.1. The 48 kDa band was successfully blocked by incubation with the immunizing peptide.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Notes

Please note, this antibody is considered Innovators Grade. Innovators Grade antibodies are generally unvalidated and require additional characterization for most new species/applications. Novus has made these antibodies available through our Innovators Reward program. Complete an online review with image, detailing your positive or negative results. In return, you receive a discount voucher for 100% of the purchase price of the reviewed product. Please contact us at innovators@novusbio.com for more details.

Alternate Names for NMNAT3 Antibody

• EC 2.7.7.1
• EC 2.7.7.18
• NaMN adenylyltransferase 3
• nicotinamide mononucleotide adenylyltransferase 3
• nicotinamide nucleotide adenylyltransferase 3
• Nicotinate-nucleotide adenylyltransferase 3
• PNAT-3
• PNAT3NMN adenylyltransferase 3
• Pyridine nucleotide adenylyltransferase 3

Background

The coenzyme NAD and its derivatives are involved in hundreds of metabolic redox reactions and are utilized in protein ADP-ribosylation, histone deacetylation, and in some Ca(2+) signaling pathways. NMNAT (EC 2.7.7.1) is a central enzyme in NAD biosynthesis, catalyzing the condensation of nicotinamide mononucleotide (NMN) or nicotinic acid mononucleotide (NaMN) with the AMP moiety of ATP to form NAD or NaAD (Zhang et al., 2003 [PubMed 12574164]).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for NMNAT3 Antibody (NB100-93455)(1)

Publication using NB100-93455

• Sorci L, Cimadamore F, Scotti S et al. Initial-rate kinetics of human NMN-adenylyltransferases: substrate and metal ion specificity, inhibition by products and multisubstrateanalogues, and isozyme contributions to NAD+ biosynthesis. Biochemistry 2007-04-24 [PMID: 17402747]

Bioinformatics

• Gene Symbol: NMNAT3
• Entrez:
  • 349565(Human)
• Uniprot:
  • Q96T66(Human)