NHC Overexpression Lysate


Western Blot: NHC Overexpression Lysate (Adult Normal) [NBL1-11621] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for NHC.

Product Details

Reactivity HuSpecies Glossary
Applications WB

Order Details

NHC Overexpression Lysate Summary


NHC Transient Overexpression Lysate

Expression Host: HEK293T

Plasmid: RC201589

Accession#: NM_006353

Protein Tag: C-MYC/DDK

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul). Each vial of cell lysate contains 100ug of total protein (at 1 mg/ml). The 2xSDS Sample Buffer consists of 4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT.


  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot.

Each vial of cell lysate contains 100ug of total protein which should be sufficient for 20-50 reactions. Depending on over-expression level, antibody affinity and detection system, some lysates can go as low as 0.1 ug per load. We recommend starting with 5ug of cell lysate. Add an equal amount of cell lysate and 2X SDS Sample buffer and boil the SDS samples for 10 minutes before loading.
Theoretical MW
9.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Store at -80C. Avoid freeze-thaw cycles.
RIPA buffer

Lysate Details for Array



HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for NHC Overexpression Lysate

  • high mobility group nucleosomal binding domain 4
  • high mobility group protein N4
  • high-mobility group (nonhistone chromosomal) protein 17-like 3
  • high-mobility group protein 17-like 3
  • HMG17L3
  • MGC5145
  • NHChigh mobility group nucleosome-binding domain-containing protein 4
  • Non-histone chromosomal protein HMG-17-like 3
  • Non-histone chromosomal protein


The protein encoded by the HMGN4 gene, a member of the HMGN protein family, is thought to reduce the compactness of thechromatin fiber in nucleosomes, thereby enhancing transcription from chromatin templates. Transcript variantsutilizing alternative polyadenylation signals exist for this gene. (provided by RefSeq)


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Gene Symbol HMGN4