NHC Overexpression Lysate Summary
NHC Transient Overexpression Lysate
Expression Host: HEK293T
Protein Tag: C-MYC/DDK
You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul). Each vial of cell lysate contains 100ug of total protein (at 1 mg/ml). The 2xSDS Sample Buffer consists of 4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT.
This product is intended for use as a positive control in Western Blot.
Each vial of cell lysate contains 100ug of total protein which should be sufficient for 20-50 reactions. Depending on over-expression level, antibody affinity and detection system, some lysates can go as low as 0.1 ug per load. We recommend starting with 5ug of cell lysate. Add an equal amount of cell lysate and 2X SDS Sample buffer and boil the SDS samples for 10 minutes before loading.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Packaging, Storage & Formulations
Store at -80C. Avoid freeze-thaw cycles.
Lysate Details for Array
HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.
Alternate Names for NHC Overexpression Lysate
- high mobility group nucleosomal binding domain 4
- high mobility group protein N4
- high-mobility group (nonhistone chromosomal) protein 17-like 3
- high-mobility group protein 17-like 3
- NHChigh mobility group nucleosome-binding domain-containing protein 4
- Non-histone chromosomal protein HMG-17-like 3
- Non-histone chromosomal protein
The protein encoded by the HMGN4 gene, a member of the HMGN protein family, is thought to reduce the compactness of thechromatin fiber in nucleosomes, thereby enhancing transcription from chromatin templates. Transcript variantsutilizing alternative polyadenylation signals exist for this gene. (provided by RefSeq)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed
for 6 months from date of receipt.
Customers Who Viewed This Item Also Viewed...
Applications: ChIP, ICC/IF, IHC, IHC-P, WB
Species: Ch, Hu, Mu, Rt
Applications: ELISA, ICC/IF, IHC, IHC-P, WB
Species: Ba, Hu, Pm, Mu, Rt
Applications: DB, ELISA, IB, ICC/IF, IHC-FrFl, IHC, IHC-Fr, IHC-P, IP, WB
Species: Hu, Mu, Rt
Applications: ELISA, IHC, IHC-P, WB
Applications: ELISA, AP, PA, WB
Species: Hu, Mu
Applications: ICC, IHC
Publications for NHC Lysate (NBL1-11621) (0)
There are no publications for NHC Lysate (NBL1-11621).
By submitting your publication information earn gift cards and discounts for future purchases.
Reviews for NHC Lysate (NBL1-11621) (0)
There are no reviews for NHC Lysate (NBL1-11621).
By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
- Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
- Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
Product General Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
FAQs for NHC Lysate (NBL1-11621) (0)
Additional NHC Products
Blogs on NHC