NHC Overexpression Lysate Summary
Transient overexpression lysate of high mobility group nucleosomal binding domain 4 (HMGN4)
The lysate was created in HEK293T cells, using Plasmid ID RC201589 and based on accession number NM_006353. The protein contains a C-MYC/DDK Tag.
This product is intended for use as a positive control in Western Blot.
You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Packaging, Storage & Formulations
Store at -80C. Avoid freeze-thaw cycles.
Lysate Details for Array
HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.
Alternate Names for NHC Overexpression Lysate
- high mobility group nucleosomal binding domain 4
- high mobility group protein N4
- high-mobility group (nonhistone chromosomal) protein 17-like 3
- high-mobility group protein 17-like 3
- NHChigh mobility group nucleosome-binding domain-containing protein 4
- Non-histone chromosomal protein HMG-17-like 3
- Non-histone chromosomal protein
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed
for 6 months from date of receipt.
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