NECAP2 Antibody

Images

 
Western Blot: NECAP2 Antibody [NBP1-84626] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp
Immunocytochemistry/ Immunofluorescence: NECAP2 Antibody [NBP1-84626] - Staining of human cell line U-2 OS shows localization to endoplasmic reticulum.
Immunohistochemistry-Paraffin: NECAP2 Antibody [NBP1-84626] - Staining of human kidney shows moderate cytoplasmic positivity in cells in tubules.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

NECAP2 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids:ANMKKKEGAAGNPRVRPASTGGLSLLPPPPGGKTSTLIPPPGEQLAVGGSLVQPAVAPSSGGAPVPWPQPNPATADIWGDFTKSTGSTSSQ
Specificity
Specificity of antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NECAP2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:500
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:20-1:50
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
NECAP2 Protein (NBP1-84626PEP)
Publications
Read Publication using NBP1-84626.

Reactivity Notes

Reactivity reported in scientific literature (PMID: 23435261)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for NECAP2 Antibody

  • adaptin ear-binding coat-associated protein 2
  • adaptin-ear-binding coat-associated protein 2
  • FLJ10420
  • NECAP endocytosis associated 2
  • NECAP endocytosis-associated protein 2
  • NECAP-2

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for NECAP2 Antibody (NBP1-84626)(1)

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

Additional NECAP2 Products

Bioinformatics Tool for NECAP2 Antibody (NBP1-84626)

Discover related pathways, diseases and genes to NECAP2 Antibody (NBP1-84626). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Pathways for NECAP2 Antibody (NBP1-84626)

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Blogs on NECAP2

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Calculators

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Bioinformatics

Gene Symbol NECAP2