MRPL45 Antibody - BSA Free

Immunohistochemistry-Paraffin: MRPL45 Antibody [NBP1-82764] - Staining of human kidney, liver, prostate and small intestine using Anti-MRPL45 antibody NBP1-82764 (A) shows similar protein distribution across tissues to independent antibody NBP1-82763 (B).

Western Blot: MRPL45 Antibody [NBP1-82764] - Analysis using Anti-MRPL45 antibody NBP1-82764 (A) shows similar pattern to independent antibody NBP1-82763 (B).

Immunocytochemistry/Immunofluorescence: MRPL45 Antibody [NBP1-82764] - Staining of human cell line U-2 OS shows localization to mitochondria. Antibody staining is shown in green.

Immunohistochemistry-Paraffin: MRPL45 Antibody [NBP1-82764] - Staining of human small intestine strong granular cytoplasmic positivity in glandular cells.

Immunohistochemistry-Paraffin: MRPL45 Antibody [NBP1-82764] - Staining of human kidney shows strong granular cytoplasmic positivity in cells in tubules.

Immunohistochemistry-Paraffin: MRPL45 Antibody [NBP1-82764] - Staining of human liver shows moderate granular cytoplasmic positivity in hepatocytes.

Immunohistochemistry-Paraffin: MRPL45 Antibody [NBP1-82764] - Staining of human prostate shows moderate granular cytoplasmic positivity in glandular cells.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

MRPL45 Antibody - BSA Free Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: VLEYVVFEKQLTNPYGSWRMHTKIVPPWAPPKQPILKTVMIPGPQLKPEEEYEEAQGEAQKPQLA
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MRPL45
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  MRPL45 Protein (NBP1-82764PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (88%), Rat (86%)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for MRPL45 Antibody - BSA Free

• L45mt
• MGC11321
• mitochondrial ribosomal protein L45
• MRP-L45,39S ribosomal protein L45, mitochondrial

Background

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within themitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. Theyhave an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed.Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA.Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes inbiochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein.Pseudogenes corresponding to this gene are found on chromosomes 2p and 17q. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MRPL45 Antibody (NBP1-82764)(1)

Publication using NBP1-82764

• Lavie J, Lalou C, Mahfouf W et al. The E3 ubiquitin ligase FBXL6 controls the quality of newly synthesized mitochondrial ribosomal proteins Cell reports 2023-05-31 [PMID: 37267103] (WB, Human)

Bioinformatics

• Gene Symbol: MRPL45