MRPL44 Antibody - Azide and BSA Free

Western Blot: MRPL44 Antibody [NBP2-96998] - Anti-MRPL44 rabbit polyclonal antibody at 1:500 dilution. Lane A: 293 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 38 kDa. Observed band size: 38 kDa

Immunohistochemistry-Paraffin: MRPL44 Antibody [NBP2-96998] - Immunochemical staining of human MRPL44 in human skeletal muscle with rabbit polyclonal antibody (1:300, formalin-fixed paraffin embedded sections).

Immunoprecipitation: MRPL44 Antibody [NBP2-96998] - Mouse MRPL44 was immunoprecipitated using: Lane A: 0.5 mg Hela Whole Cell Lysate. Lane B: 0.5 mg 293T Whole Cell Lysate. Lane C: 0.5 mg Caco-2 Whole Cell Lysate. 4 ul anti-Mouse MRPL44 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-Mouse MRPL44 rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 38 kDa. Observed band size: 38 kDa

Immunocytochemistry/Immunofluorescence: MRPL44 Antibody [NBP2-96998] - Immunofluorescence staining of MRPL44 in HUVEC cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-96998 antibody (dilution ratio 1:300) at 4C overnight. Then cells were stained with the Alexa Fluor$488-conjugated Goat Anti-rabbit IgG Secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to cytoplasm.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

MRPL44 Antibody - Azide and BSA Free Summary

• Description: This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
• Immunogen: Produced in rabbits immunized with purified, recombinant Human MRPL44 (Uniprot#: Q9H9J2; Met1-Ser332)
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MRPL44
• Purity: Antigen and protein A Affinity-purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:5000-1:10000
  • Immunocytochemistry/ Immunofluorescence 1:200-1:1000
  • Immunohistochemistry-Paraffin 1:200-1:500
  • Immunoprecipitation 4-8 uL/mg of lysate
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.2 um filtered solution in PBS
• Preservative: No Preservative
• Purity: Antigen and protein A Affinity-purified

Alternate Names for MRPL44 Antibody - Azide and BSA Free

• 39S ribosomal protein L44, mitochondrial
• EC 3.1.26
• EC 3.1.26.-
• FLJ12701
• FLJ13990
• FLJ37688
• L44MT
• mitochondrial ribosomal protein L44
• MRP-L44

Background

MRPL44, or Mitochondrial Ribosomal Protein L44, consists of a 332 amino acid isoform that is 38 kDa, and is involved in protein synthesis as a part of the 39S mitochondrial ribosome subunit. Disease research is currently being conducted to determine the relationship between MRPL44 and intrahepatic cholangiocarcinoma. The protein has been linked to RNA processing, and interacts with IMMT, SETDB1, VIM, USP22, and CEP70.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MRPL44