MFAP3L Antibody (1059107) [Unconjugated]

MFAP3L was detected in immersion fixed RT‑4 human urinary bladder transitional cell papilloma cell line (Positive) & absent in HL‑60 human acute promyelocytic leukemia cell line (Negative) using Mouse Anti-Human MFAP3L Monoclonal Antibody (Catalog # MAB11325) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

MFAP3L was detected in immersion fixed RT‑4 human urinary bladder transitional cell papilloma cell line (Positive) & absent in THP‑1 human acute monocytic leukemia cell line (Negative) using Mouse Anti-Human MFAP3L Monoclonal Antibody (Catalog # MAB11325) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

MFAP3L was detected in immersion fixed SK‑BR‑3 human breast cancer cell line (Positive) & absent in HL‑60 human acute promyelocytic leukemia cell line (Negative) using Mouse Anti-Human MFAP3L Monoclonal Antibody (Catalog # MAB11325) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

MFAP3L was detected in immersion fixed SK‑BR‑3 human breast cancer cell line (Positive) & absent in THP‑1 human acute monocytic leukemia cell line (Negative) using Mouse Anti-Human MFAP3L Monoclonal Antibody (Catalog # MAB11325) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

• Reactivity: Hu
• Applications: ICC/IF
• Clone: 1059107
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

MFAP3L Antibody (1059107) [Unconjugated] Summary

• Immunogen: Human embryonic kidney cell, HEK293-derived human MFAP3L
  Met1-Met149
  Accession # O75121
• Specificity: Detects human MFAP3L in direct ELISA.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 8-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for MFAP3L Antibody (1059107) [Unconjugated]

• HSD39
• HSD-39
• MFAP3L
• microfibrillar-associated protein 3-like
• NYD-sp9
• testis development protein NYD-SP9

Background

Microbibrillar-Associated Protein 3-Like (MFAP3L), also known as NYD-sp9, is part of the microfibrillar-associated protein family (MFAPs). MFAPs are non‐fibrillin, extracellular matrix glycoproteins that interact with fibrillin and were originally characterized in microfibrillar assembly (1,2). In humans, there several subfamily members with varying amino acid (aa) sequence homology and functions (1,2). Among the family, MFAP2 and MFAP5 are more closely related and while MFAP1, 3 and 4 share no structural or sequence homology with MFAP2, MFAP5 or with each other (1,2). Human MFAP3L shows 71% amino acid (aa) sequence homology to MFAP3, but not other MFAPs (3). Mature, human MFAP3L consists of an extracellular domain (ECD) containing N-linked glycosylation sites, a transmembrane domain, and a cytoplasmic domain with a conserved SH2 motif (3). The ECD of human MFAP3L shares 89% and 90% aa sequence identity with mouse and rat MFAP3L, respectively. MFAPs have the unique ability to interact with TGF-beta family growth factors, Notch and Notch ligands and multiple elastic fiber proteins, in addition to interacting with fibrillin (1, 2). MFAPs are expressed in a wide variety of tissues and, along with microfibril assembly, they play roles in the regulation of tissue homeostasis, cell survival, and tumor progression (1,2). MFAP3L is often located within colorectal cancer (CRC) cells, which metastasize by activation of the nuclear ERK pathway via MFAP3L phosphorylation (3). Regulation of this MFAP3L activity could have pharmaceutical effects on CRC tumor progression (3).

1. Zhu, S. et al. (2020) J Cell Physio. 236:41.
2. Mecham, R.P. et al. (2015) Matrix Biol. 47:13.
3. Lou, X. et al. (2014) BBA. 1842:1423.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • O75121()